Astrocyte Markers Antibody Sampler Kit
cat.: HAK21083
Product Type: Antibody Sampler Kit
Species reactivity: Mouse
Applications: WB
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Uniprot #: P14136 P43003 O15392 P43004 O75891 P55087 P30531 P03995 P56564 O70201 P43006 Q8R0Y6 P55088 P31648 P47819 P24942 Q9JHY7 P31596 P28037 P47863 P23978
Images
HAK21083_1.jpg Fig1: Western blot analysis of Aquaporin 4 on different lysates with Rabbit anti-Aquaporin 4 antibody (HA722672) at 1/2,000 dilution.

Lane 1: Mouse brain tissue lysate (no heat) (40 µg/Lane)
Lane 2: Mouse liver tissue lysate (negative) (40 µg/Lane)
Lane 3: Rat brain tissue lysate (no heat) (40 µg/Lane)
Lane 4: Rat liver tissue lysate (negative) (40 µg/Lane)

Notice: no heat means the lysate is not boiled.

Predicted band size: 35 kDa
Observed band size: 30 kDa

Exposure time: 16 seconds; ECL: K1801;
4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722672) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
HAK21083_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-Aquaporin 4 antibody (HA722672) at 1/10,000 dilution and competitor's antibody at 1/2,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722672) at 1/10,000 dilution and competitor's antibody at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HAK21083_3.jpg Fig3: Western blot analysis of GABA Transporter 1 / GAT 1 on different lysates with Rabbit anti-GABA Transporter 1 / GAT 1 antibody (HA721496) at 1/1,000 dilution.

Lane 1: Mouse cerebellum tissue lysate
Lane 2: Mouse cerebellum tissue lysate (70℃ heat)
Lane 3: Mouse brain tissue lysate
Lane 4: Mouse brain tissue lysate (70℃ heat)
Lane 5: Rat brain tissue lysate
Lane 6: Rat brain tissue lysate (70℃ heat)

Lysates/proteins at 30 µg/Lane.

Predicted band size: 67 kDa
Observed band size: 67 kDa

Exposure time: Lane 1-4: 1 minute 40 seconds; Lane 5-6: 5 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721496) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
HAK21083_4.jpg Fig4: Immunofluorescence analysis of frozen mouse cerebellum tissue with Rabbit anti-GABA Transporter 1 / GAT 1 antibody (HA721496) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA721496, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
HAK21083_5.jpg Fig5: Fluorescence multiplex immunohistochemical analysis of mouse hippocampus (Formalin/PFA-fixed paraffin-embedded sections). Panel A: the merged image of anti-GFAP (ET1601-23, Green), anti-NeuN (ET1602-12, Red) and anti-c-Fos (HA722666, White) on hippocampus. HRP Conjugated UltraPolymer Goat Polyclonal Antibody HA1119/HA1120 was used as a secondary antibody. The immunostaining was performed with the Sequential Immuno-staining Kit (IRISKit™MH010101, www.luminiris.cn). The section was incubated in three rounds of staining: in the order of ET1601-23 (1/1,000 dilution), ET1602-12 (1/1,000 dilution) and HA722666 (1/200 dilution) for 20 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Zeiss Observer 7 Inverted Fluorescence Microscope.
HAK21083_6.jpg Fig6: Fluorescence multiplex immunohistochemical analysis of mouse brain (Formalin/PFA-fixed paraffin-embedded sections). Panel A: the merged image of anti-GFAP (ET1601-23, Green), anti-NeuN (ET1602-12, Red) and anti-c-Fos (HA722666, White) on brain. HRP Conjugated UltraPolymer Goat Polyclonal Antibody HA1119/HA1120 was used as a secondary antibody. The immunostaining was performed with the Sequential Immuno-staining Kit (IRISKit™MH010101, www.luminiris.cn). The section was incubated in three rounds of staining: in the order of ET1601-23 (1/1,000 dilution), ET1602-12 (1/1,000 dilution) and HA722666 (1/200 dilution) for 20 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Zeiss Observer 7 Inverted Fluorescence Microscope.
HAK21083_7.jpg Fig7: Fluorescence multiplex immunohistochemical analysis of mouse hippocampus (Formalin/PFA-fixed paraffin-embedded sections). Panel A: the merged image of anti-MAP2 (HA500177, Green), anti-GFAP (ET1601-23, Red) and anti-NeuN (ET1602-12, Magenta) on Mouse hippocampus. HRP Conjugated UltraPolymer Goat Polyclonal Antibody HA1119/HA1120 was used as a secondary antibody. The immunostaining was performed with the Sequential Immuno-staining Kit (IRISKit™MH010101, www.luminiris.cn). The section was incubated in three rounds of staining: in the order of HA500177 (1/1,000 dilution), ET1601-23 (1/1,000 dilution) and ET1602-12 (1/10,000 dilution) for 20 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
HAK21083_8.jpg Fig8: Aquaporin 4 was immunoprecipitated from 0.2 mg mouse brain tissue lysate with HA722672 at 2 µg/25 µl agarose. Western blot was performed from the immunoprecipitate using HA722672 at 1/1,000 dilution. Mouse anti Rabbit IgG heavy chain (Fc) secondary antibody (M1003-7) at 1/100,000 dilution was used for 1 hour at room temperature.

Lane 1: Mouse brain tissue lysate (input)
Lane 2: HA722672 IP in mouse brain tissue lysate
Lane 3: Rabbit IgG instead of HA722672 in mouse brain tissue lysate

Blocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 1 minute; ECL: K1801
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.