LRP1-mediated Endocytosis and Transmission of Tau Antibody Sampler Kit
cat.: HAK21098
| Product Type: | Antibody Sampler Kit |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IHC-P |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Uniprot #: | SwissProt: P17879 Mouse | Q61696 Mouse | P0DMV9 Human | P0DMV8 Human | Q07439 Rat | P02649 Human | P08226 Mouse | P10636 Human | P10637 Mouse | P19332 Rat | P10636-8 Human | P10637 Mouse | P10636-8 Human | P10637 Mouse | P19332 Rat | Q92673 Human | O88307 Mouse | Q9R0N2 Rat | P20339 Human | Q9CQD1 Mouse | M0RC99 Rat | P51149 Human | P51150 Mouse | P09527 Rat | P62491 Human | P62492 Mouse | P62494 Rat |
Images
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Fig1:
Western blot analysis of Hsp70 on different lysates with Rabbit anti-Hsp70 antibody (ET1601-11) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: A549 cell lysate Lane 3: MCF7 cell lysate Lane 4: HCT 116 cell lysate Lane 5: Mouse brain tissue lysate Lane 6: Mouse testis tissue lysate Lane 7: Rat testis tissue lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 70 kDa Observed band size: 70 kDa Exposure time: Lane 1-4: 43 seconds; Lane 5-7: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1601-11) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-Hsp70 antibody (ET1601-11) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1601-11) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue with Rabbit anti-Hsp70 antibody (ET1601-11) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1601-11) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Western blot analysis of Apolipoprotein E on human plasma tissue lysates with Rabbit anti-Apolipoprotein E antibody (ET1610-22) at 1/1,000 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 36 kDa Observed band size: 33 kDa Exposure time: 6 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1610-22) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded AD mouse brain tissue with Rabbit anti-Apolipoprotein E antibody (ET1610-22) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-22) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Immunofluorescence analysis of frozen mouse cerebral cortex tissue labeling Tau with Rabbit anti-Tau antibody (ET1603-2). The tissues were blocked in 3% BSA for 30 minutes at room temperature, washed with PBS, and then probed with the primary antibody (ET1603-2, green) at 1/50 dilution overnight at 4℃, washed with PBS. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue). Image acquisition was performed with KFBIO KF-FL-400 Scanner. |
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Fig7:
Immunofluorescence analysis of frozen mouse hippocampus tissue labeling Tau with Rabbit anti-Tau antibody (ET1603-2). The tissues were blocked in 3% BSA for 30 minutes at room temperature, washed with PBS, and then probed with the primary antibody (ET1603-2, green) at 1/50 dilution overnight at 4℃, washed with PBS. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue). Image acquisition was performed with KFBIO KF-FL-400 Scanner. |
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Fig8:
Tau was immunoprecipitated from 0.2 mg mouse brain tissue lysate with ET1603-2 at 2 µg/25 µl agarose. Western blot was performed from the immunoprecipitate using ET1603-2 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: Mouse brain tissue lysate (input) Lane 2: ET1603-2 IP in mouse brain tissue lysate Lane 3: Rabbit IgG instead of ET1603-2 in mouse brain tissue lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 3 seconds; ECL: K1801 |
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Fig9:
Western blot analysis of Phospho-Tau (T181) on different lysates with Rabbit anti-Phospho-Tau (T181) antibody (HA722271) at 1/1,000 dilution. Lane 1: Human brain tissue lysate Lane 2: Human brain tissue lysate, the membrane treated with λpp for 1 hour Lysates/proteins at 40 µg/Lane. Predicted band size: 79 kDa Observed band size: 70 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722271) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig10:
Western blot analysis of Phospho-Tau (S404) on different lysates with Rabbit anti-Phospho-Tau (S404) antibody (HA721801) at 1/1,000 dilution. Lane 1: Human brain tissue lysate Lane 2: Mouse brain tissue lysate Lane 3: Rat brain tissue lysate Lane 4: Mouse brain tissue lysate, the membrane treated with λpp for 1 hour Lysates/proteins at 30 µg/Lane. Predicted band size: 79 kDa Observed band size: 50-70 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721801) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig11:
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Phospho-Tau (S404) antibody (HA721801) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721801) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig12:
Western blot analysis of Rab5 on different lysates with Rabbit anti-Rab5 antibody (ET1609-27) at 1/1,000 dilution. Lane 1: MCF-7 cell lysate Lane 2: Hela cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 24 kDa Observed band size: 24 kDa Exposure time: 1 minute; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1609-27) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
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Fig13:
Western blot analysis of RAB7 on different lysates with Rabbit anti-RAB7 antibody (ET1611-96) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: MCF7 cell lysate Lane 3: Neuro-2a cell lysate Lane 4: C2C12 cell lysate Lane 5: PC-12 cell lysate Lane 6: C6 cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 23 kDa Observed band size: 23 kDa Exposure time: 17 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-96) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig14:
Immunocytochemistry analysis of C6 cells labeling RAB7 with Rabbit anti-RAB7 antibody (ET1611-96) at 1/1,000 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-RAB7 antibody (ET1611-96) at 1/1,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig15:
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-RAB7 antibody (ET1611-96) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-96) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig16:
Western blot analysis of Rab11A on different lysates with Rabbit anti-Rab11A antibody (HA721552) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: A549 cell lysate (20 µg/Lane) Lane 3: SH-SY5Y cell lysate (20 µg/Lane) Lane 4: Neuro-2a cell lysate (20 µg/Lane) Lane 5: C6 cell lysate (20 µg/Lane) Lane 6: Mouse testis tissue lysate (40 µg/Lane) Lane 7: Rat testis tissue lysate (40 µg/Lane) Lane 8: Rat brain tissue lysate (40 µg/Lane) Predicted band size: 24 kDa Observed band size: 24 kDa Exposure time: 2 minutes 37 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721552) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
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Fig17:
Immunohistochemical analysis of paraffin-embedded human small intestine tissue with Rabbit anti-Rab11A antibody (HA721552) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721552) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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