Synaptic Marker Antibody Sampler Kit
cat.: HAK21178
| Product Type: | Antibody Sampler Kit |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Uniprot #: | SwissProt: P08247 Human | Q62277 Mouse | P07825 Rat | P17600 Human | Q92777 Human | O88935 Mouse | Q64332 Mouse | P09951 Rat | Q63537 Rat | P78352 Human | Q62108 Mouse | P31016 Rat | P63027 Human | P63044 Mouse | P63045 Rat |
Images
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Fig1:
Western blot analysis of Synaptophysin on different lysates with Rabbit anti-Synaptophysin antibody (ET1606-56) at 1/5,000 dilution. Lane 1: SH-SY5Y cell lysate Lane 2: HeLa cell lysate (negative) Lane 3: NIH/3T3 cell lysate (negative) Lane 4: PC-12 cell lysate Lane 5: Human brain tissue lysate Lane 6: Mouse brain tissue lysate Lane 7: Rat brain tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 34 kDa Observed band size: 34/40 kDa Exposure time: 43 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1606-56) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Application: IHC-Fr Species: Mouse Site: Cerebellum Sample: Frozen section Antibody concentration: 1/1,000 Antigen retrieval: Not required |
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Fig3:
Western blot analysis of Synapsin I + II on different lysates with Rabbit anti-Synapsin I + II antibody (HA723196) at 1/5,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Mouse lung tissue lysate (negative) Lane 3: Rat brain tissue lysate Lane 4: Rat lung tissue lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 74 kDa Observed band size: 50-74 kDa Exposure time: 6 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723196) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig4:
Immunocytochemistry analysis of mouse primary neuronal cells labeling Synapsin I + II with Rabbit anti-Synapsin I + II antibody (HA723196) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Synapsin I + II antibody (HA723196) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig5:
Application: IHC-Fr Species: Mouse Site: Cerebellum Sample: Frozen section Antibody concentration: 1:500 Antigen retrieval: The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. |
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Fig6:
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Synapsin I + II antibody (HA723196) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723196) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig7:
Synapsin I + II was immunoprecipitated from 0.2 mg mouse brain tissue lysate with HA723196 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA723196 at 1/1,000 dilution. Mouse Anti-Rabbit IgG kappa light chain secondary antibody (M1208-2) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: mouse brain tissue lysate (input) Lane 2: HA723196 IP in mouse brain tissue lysate Lane 3: Rabbit IgG instead of HA723196 in mouse brain tissue lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 3 seconds; ECL: K1801 |
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Fig8:
Application: IHC-Fr Species: Mouse Site: Cerebellum Sample: Frozen section Antibody concentration: 1: 500 (PSD95, ET1602-20, red); 1:500 (Iba1, HA601376, green) Antigen retrieval: Not required |
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Fig9:
Application: IHC-Fr Species: Rat Site: Cerebellum Sample: Frozen section Antibody concentration: 1: 1,000 (PSD95, ET1602-20, red); 1:500 (VGLUT2, HA601393, green) Antigen retrieval: Not required |
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Fig10:
Western blot analysis of PSD95 on different lysates with Rabbit anti-PSD95 antibody (ET1602-20) at 1/2,000 dilution. Lane 1: Human brain tissue lysate (20 µg/Lane) Lane 2: Mouse brain tissue lysate (20 µg/Lane) Lane 3: Mouse hippocampus tissue lysate (20 µg/Lane) Lane 4: Rat brain tissue lysate (20 µg/Lane) Lane 5: Rat hippocampus tissue lysate (20 µg/Lane) Predicted band size: 80 kDa Observed band size: 75-100 kDa Exposure time: 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1602-20) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig11:
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-PSD95 antibody (ET1602-20) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1602-20) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig12:
Western blot analysis of VAMP2 on different lysates with Rabbit anti-VAMP2 antibody (ET1703-50) at 1/1,000 dilution. Lane 1: Human brain tissue lysate Lane 2: Mouse brain tissue lysate Lane 3: Rat brain tissue lysate Lysates/proteins at 40 µg/Lane. Predicted band size: 13 kDa Observed band size: 18 kDa Exposure time: 2 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-50) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig13:
Immunocytochemistry analysis of U-87 MG cells labeling VAMP2 with Rabbit anti-VAMP2 antibody (ET1703-50) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-VAMP2 antibody (ET1703-50) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig14:
Immunocytochemistry analysis of C6 cells labeling VAMP2 with Rabbit anti-VAMP2 antibody (ET1703-50) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-VAMP2 antibody (ET1703-50) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig15:
Application: IF-tissue Species: Mouse Site: Cerebellum Sample: Paraffin-embedded section Antibody concentration: 1/500 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.