CD11c Recombinant Rabbit Monoclonal Antibody
cat.: IRS001
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: mIHC
Clonality: Monoclonal
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer: PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Purification: Protein A affinity purified.
Isotype: IgG
Immunogen: Synthetic peptide within Human CD11c aa 1,114-1,163 / 1,163 (Cytoplasmic).
Positive control: Human cholangiocarcinoma tissue, human tonsil tissue, human colon tissue, human kidney tissue.
Subcellular location: Membrane.
Recommended Dilutions:
  mIHC
1:100. This antibody has been validated for multiplex staining with LUMINIRIS mTSA, supporting both multiplex and super-multiplex staining.
Uniprot #: SwissProt: P20702 Human
Alternative names: 95 alpha chain 95 CD 11c CD11 antigen-like family member C CD11c CD11c antigen Complement component 3 receptor 4 subunit CR4 Integrin alpha X Integrin alpha X chain Integrin alpha-X Integrin aX Integrin subunit alpha X integrin, alpha X (antigen CD11C (p150), alpha polypeptide) integrin, alpha X (complement component 3 receptor 4 subunit ITAX_HUMAN ITGAX LEU M5 alpha subunit Leu M5 Leukocyte adhesion glycoprotein p150 95 alpha chain Leukocyte adhesion glycoprotein p150 Leukocyte adhesion receptor p150 95 Leukocyte adhesion receptor p150 Leukocyte surface antigen p150 95 alpha subunit Leukocyte surface antigen p150 alpha subunit Myeloid membrane antigen alpha subunit p150 95 integrin alpha chain p150 p150/95 SLEB6
Images
IRS001_1.jpg Fig1: mIHC analysis of human cholangiocarcinoma tissue (Formalin/PFA-fixed paraffin-embedded sections) with CD11c (IRS001), CD20 (IRS003), CD8 and panCK (IRS010) antibody at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900802). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
IRS001_2.jpg Fig2: mIHC analysis of human cholangiocarcinoma tissue (Formalin/PFA-fixed paraffin-embedded sections) with CD11c (IRS001), CD68 (IRS004), CD20 (IRS003), CD38 (IRS006), CD8 and panCK (IRS010) antibody at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900802). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
IRS001_3.jpg Fig3: mIHC analysis of human tonsil tissue (Formalin/PFA-fixed paraffin-embedded sections) with CD8, CD4 (IRS005), FOXP3, CD68 (IRS004), CD38 (IRS006), CD11c (IRS001), PD-L1, CD163 (IRS002) and CD20 (IRS003) antibody at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900802). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
IRS001_4.jpg Fig4: mIHC analysis of human tonsil tissue (Formalin/PFA-fixed paraffin-embedded sections) with Ki67 (IRS036), CD11c (IRS001), PD-1 and CD8 (IRS007) antibody at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900802). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
IRS001_5.jpg Fig5: mIHC analysis of human tonsil tissue (Formalin/PFA-fixed paraffin-embedded sections) with CD38 (IRS006), CD57 (IRS027), CD11c (IRS001), Tryptase (IRS041) and CD45 (IRS025) antibody at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900802). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
IRS001_6.jpg Fig6: mIHC analysis of human tonsil tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-CD11c antibody (IRS001) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900808). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
IRS001_7.jpg Fig7: mIHC analysis of human colon tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-CD11c antibody (IRS001) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900808). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
IRS001_8.jpg Fig8: mIHC analysis of human craniopharyngioma tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-CD11c antibody (IRS001) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900808). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
IRS001_9.jpg Fig9: mIHC analysis of human kidney tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-CD11c antibody (IRS001) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900808). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.