CD20 Recombinant Rabbit Monoclonal Antibody
cat.: IRS003
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: mIHC
Clonality: Monoclonal
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Purification: Protein A affinity purified.
Isotype: IgG
Immunogen: Synthetic peptide within Human CD20 aa 210-297 (Cytoplasmic).
Positive control: Human tonsil tissue, human lung cancer with brain metastasis tissue, human cervical cancer tissue, human gastric cancer tissue, human small cell lung cancer tissue, human pancreatic cancer tissue, human lung tissue, human liver tissue, human spleen tissue.
Subcellular location: Cell membrane.
Recommended Dilutions:
  mIHC
1:100. This antibody has been validated for multiplex staining with LUMINIRIS mTSA, supporting both multiplex and super-multiplex staining.
Uniprot #: SwissProt: P11836 Human
Alternative names: APY ATOPY B lymphocyte antigen CD20 B Lymphocyte Cell Surface Antigen B1 B-lymphocyte antigen CD20 B-lymphocyte cell-surface antigen B1 B-lymphocyte surface antigen B1 B1 Bp 35 Bp35 CD 20 CD20 CD20 antigen CD20 receptor CD20_HUMAN CVID 5 CVID5 Fc epsilon receptor I beta chain Fc Fragment of IgE high affinity I receptor for beta polypeptide FCER1B High affinity immunoglobulin epsilon receptor subunit beta IgE Fc receptor subunit beta IGEL IGER IGHER LEU 16 Leu-16 LEU16 leukocyte surface antigen Leu 16 Leukocyte surface antigen Leu-16 Ly44 Membrane spanning 4 domains A1 Membrane spanning 4 domains subfamily A member 2 membrane-spanning 4-domains A1 Membrane-spanning 4-domains subfamily A member 1 MGC3969 MS4A1 MS4A2 S7
Images
IRS003_1.jpg Fig1: mIHC analysis of human tonsil tissue (Formalin/PFA-fixed paraffin-embedded sections) with CD8, CD4 (IRS005), FOXP3, CD68 (IRS004), CD38 (IRS006), CD11c (IRS001), PD-L1, CD163 (IRS002) and CD20 (IRS003) antibody at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900802). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
IRS003_2.jpg Fig2: mIHC analysis of human lung cancer with brain metastasis tissue (Formalin/PFA-fixed paraffin-embedded sections) with CD3, CD20 (IRS003), panCK (IRS010), CD68 (IRS004) and Ki67 antibody at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900802). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
IRS003_3.jpg Fig3: mIHC analysis of human cervical cancer tissue (Formalin/PFA-fixed paraffin-embedded sections) with CD8, CD20 (IRS003), FOXP3, CK19 (IRS011), PD-L1, CD4 (IRS005), CD163 (IRS002) and CD68 (IRS004) antibody at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900802). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
IRS003_4.jpg Fig4: mIHC analysis of human colorectal cancer tissue (Formalin/PFA-fixed paraffin-embedded sections) with CD8, CD20 (IRS003), CD68 (IRS004), CD4 (IRS005) and panCK (IRS010) antibody at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900802). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
IRS003_5.jpg Fig5: mIHC analysis of human gastric cancer tissue (Formalin/PFA-fixed paraffin-embedded sections) with PD-L1, CD8, CD3, CD68 (IRS004), CD20 (IRS003) and panCK (IRS010) antibody at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900802). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
IRS003_6.jpg Fig6: mIHC analysis of human small cell lung cancer tissue (Formalin/PFA-fixed paraffin-embedded sections) with PD-L1, CD8, CD3, CD68 (IRS004), CD20 (IRS003) and panCK (IRS010) antibody at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900802). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
IRS003_7.jpg Fig7: mIHC analysis of human pancreatic cancer tissue (Formalin/PFA-fixed paraffin-embedded sections) with CD3, CD68 (IRS004), CD20 (IRS003) and panCK (IRS010) antibody at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900802). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
IRS003_8.jpg Fig8: mIHC analysis of human tonsil tissue (Formalin/PFA-fixed paraffin-embedded sections) with CD56 (IRS026), CD68 (IRS004), CD163 (IRS002), panCK (IRS010) and CD20 (IRS003) antibody at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900802). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
IRS003_9.jpg Fig9: mIHC analysis of human tonsil tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-CD20 antibody (IRS003) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900808). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
IRS003_10.jpg Fig10: mIHC analysis of human tonsil tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-CD20 antibody (IRS003) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900808). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
IRS003_11.jpg Fig11: mIHC analysis of human lung tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-CD20 antibody (IRS003) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900808). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
IRS003_12.jpg Fig12: mIHC analysis of human liver tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-CD20 antibody (IRS003) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900808). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
IRS003_13.jpg Fig13: mIHC analysis of human spleen tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-CD20 antibody (IRS003) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900808). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.