CD4 Recombinant Rabbit Monoclonal Antibody
cat.: IRS005
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: mIHC
Clonality: Monoclonal
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer: PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Purification: Protein A affinity purified.
Isotype: IgG
Immunogen: Recombinant protein within Human CD4 aa 196-416 / 458.
Positive control: Human tonsil tissue, human cervical cancer tissue, human colorectal cancer tissue, human spleen tissue, human kidney tissue.
Subcellular location: Cell membrane.
Recommended Dilutions:
  mIHC
1:100. This antibody has been validated for multiplex staining with LUMINIRIS mTSA, supporting both multiplex and super-multiplex staining.
Uniprot #: SwissProt: P01730 Human
Alternative names: CD 4 CD4 (L3T4) CD4 CD4 antigen (p55) CD4 antigen CD4 molecule CD4 receptor CD4+ Lymphocyte deficiency, included CD4_HUMAN CD4mut L3T4 Leu3 Ly-4 Lymphocyte antigen CD4 MGC165891 OTTHUMP00000238897 p55 T cell antigen T4 T cell antigen T4/LEU3 T cell differentiation antigen L3T4 T cell OKT4 deficiency, included T cell surface antigen T4/Leu 3 T cell surface antigen T4/Leu3 T cell surface glycoprotein CD4 T-cell surface antigen T4/Leu-3 T-cell surface glycoprotein CD4 W3/25 W3/25 antigen
Images
IRS005_1.jpg Fig1: mIHC analysis of human tonsil tissue (Formalin/PFA-fixed paraffin-embedded sections) with CD8, CD4 (IRS005), FOXP3, CD68 (IRS004), CD38 (IRS006), CD11c (IRS001), PD-L1, CD163 (IRS002) and CD20 (IRS003) antibody at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900802). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
IRS005_2.jpg Fig2: mIHC analysis of human cervical cancer tissue (Formalin/PFA-fixed paraffin-embedded sections) with CD8, CD20 (IRS003), FOXP3, CK19 (IRS011), PD-L1, CD4 (IRS005), CD163 (IRS002) and CD68 (IRS004) antibody at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900802). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
IRS005_3.jpg Fig3: mIHC analysis of human colorectal cancer tissue (Formalin/PFA-fixed paraffin-embedded sections) with CD8, CD20 (IRS003), CD68 (IRS004), CD4 (IRS005) and panCK (IRS010) antibody at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900802). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
IRS005_4.jpg Fig4: mIHC analysis of human tonsil tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-CD4 antibody (IRS005) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900808). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
IRS005_5.jpg Fig5: mIHC analysis of human spleen tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-CD4 antibody (IRS005) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900808). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
IRS005_6.jpg Fig6: mIHC analysis of human kidney tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-CD4 antibody (IRS005) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900808). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.