| Product Type: | Mouse monoclonal IgG2b, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | mIHC |
| Clonality: | Monoclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 26 kDa |
| Isotype: | IgG2b |
| Immunogen: | Synthesized peptide derived from human CD8 AA range: 100-235 |
| Subcellular location: | Cell membrane, Secreted. |
| Recommended Dilutions:
mIHC |
1:100 |
| Uniprot #: | SwissProt: P01732 Human |
| Alternative names: | alpha polypeptide (p32) CD8 CD8 antigen alpha polypeptide CD8 antigen alpha polypeptide (p32) CD8a CD8A antigen CD8A molecule CD8A_HUMAN Leu2 Leu2 T lymphocyte antigen Ly3 LYT3 MAL OKT8 T cell antigen OTTHUMP00000160760 OTTHUMP00000160764 OTTHUMP00000203528 OTTHUMP00000203721 p32 T cell antigen Leu2 T cell co receptor T-cell surface glycoprotein CD8 alpha chain T-lymphocyte differentiation antigen T8/Leu-2 T8 T cell antigen T8/Leu-2 T-lymphocyte differentiation antigen |
|
Fig1: mIHC analysis of human tonsil tissue (Formalin/PFA-fixed paraffin-embedded sections) with Ki67 (IRS036), CD11c (IRS001), PD-1 and CD8 (IRS007) antibody at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900802). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner. |
|
Fig2: mIHC analysis of human lung cancer tissue (Formalin/PFA-fixed paraffin-embedded sections) with FOXP3 (IRS008), CD57 (IRS027), MPO (IRS037), CD8 (IRS007) and panCK (IRS010) antibody at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900802). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner. |