Cytokeratin 19 Recombinant Rabbit Monoclonal Antibody
cat.: IRS011
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | mIHC |
| Clonality: | Monoclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Cytokeratin 19 aa 348-400 / 400. |
| Positive control: | Human cervical cancer tissue, human endometrial cancer tissue, human pancreas tissue, human cholangiocarcinoma tissue. |
| Subcellular location: | Cytoplasm |
| Recommended Dilutions:
mIHC |
1:100. This antibody has been validated for multiplex staining with LUMINIRIS mTSA, supporting both multiplex and super-multiplex staining. |
| Uniprot #: | SwissProt: P08727 Human |
| Alternative names: | 40 kDa keratin intermediate filament CK 19 CK-19 CK19 Cytokeratin 19 Cytokeratin-19 K19 K1C19_HUMAN K1CS Keratin 19 Keratin type I 40 kD Keratin type I 40kD Keratin type I cytoskeletal 19 Keratin, type I cytoskeletal 19 Keratin, type I, 40 kd Keratin-19 KRT19 MGC15366 |
Images
|
Fig1: mIHC analysis of human cervical cancer tissue (Formalin/PFA-fixed paraffin-embedded sections) with CD8, CD20 (IRS003), FOXP3, CK19 (IRS011), PD-L1, CD4 (IRS005), CD163 (IRS002) and CD68 (IRS004) antibody at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900802). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner. |
|
Fig2: mIHC analysis of human endometrial cancer tissue (Formalin/PFA-fixed paraffin-embedded sections) with PD-L1, CD8, CD3, CK19 (IRS011), FOXP3 and CD68 (IRS004) antibody at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900802). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner. |
|
Fig3: mIHC analysis of human pancreas tissue (Formalin/PFA-fixed paraffin-embedded sections) with CHGA, CD31 (IRS023), CK19 (IRS011) and Somatostatin 28 antibody at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900802). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner. |
|
Fig4: mIHC analysis of human cholangiocarcinoma tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-Cytokeratin 19 antibody (IRS011) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900808). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner. |
|
Fig5: mIHC analysis of human cholangiocarcinoma tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-Cytokeratin 19 antibody (IRS011) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900808). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner. |
|
Fig6: mIHC analysis of human pancreas tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-Cytokeratin 19 antibody (IRS011) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900808). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.