alpha smooth muscle Actin Recombinant Rabbit Monoclonal Antibody
cat.: IRS012
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: mIHC
Clonality: Monoclonal
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 42 kDa
Isotype: IgG
Immunogen: Synthetic peptide within N-terminal human alpha smooth muscle Actin.
Positive control: Human lung cancer tissue, human gastric cancer tissue, human tonsils tissue, human lung squamous cell carcinoma tissue, human liver tissue, human craniopharyngioma tissue.
Subcellular location: Cytoplasm.
Recommended Dilutions:
  mIHC
1:100
Uniprot #: SwissProt: P62736 Human
Alternative names: alpha SMA a-SMA asma a actin AAT6 ACTA_HUMAN ACTA2 Actin alpha 2 smooth muscle aorta Actin aortic smooth muscle Actin, aortic smooth muscle ACTSA ACTVS Alpha 2 actin Alpha actin 2 Alpha cardiac actin Alpha-actin-2 Cell growth inhibiting gene 46 protein Cell growth-inhibiting gene 46 protein GIG46 Growth inhibiting gene 46 MYMY5
Images
IRS012_1.jpg Fig1: mIHC analysis of human lung cancer tissue (Formalin/PFA-fixed paraffin-embedded sections) with CD34 (IRS024), panCK (IRS010), FAP and aSMA (IRS012) antibody at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900802). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
IRS012_2.jpg Fig2: mIHC analysis of human gastric cancer tissue (Formalin/PFA-fixed paraffin-embedded sections) with CD11b (IRS014), CD31 (IRS023), aSMA (IRS012), panCK (IRS010) and CD3 (IRS022) antibody at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900802). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
IRS012_3.jpg Fig3: mIHC analysis of human gastric cancer tissue (Formalin/PFA-fixed paraffin-embedded sections) with CD21 (IRS019), PD-1, CD14 (IRS015), CD38 (IRS006), aSMA (IRS012) and CK7 (IRS029) antibody at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900802). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
IRS012_4.jpg Fig4: mIHC analysis of human tonsils tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-alpha smooth muscle Actin antibody (IRS012) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900808). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
IRS012_5.jpg Fig5: mIHC analysis of human lung squamous cell carcinoma tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-alpha smooth muscle Actin antibody (IRS012) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900808). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
IRS012_6.jpg Fig6: mIHC analysis of human liver tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-alpha smooth muscle Actin antibody (IRS012) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900808). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
IRS012_7.jpg Fig7: mIHC analysis of human craniopharyngioma tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-alpha smooth muscle Actin antibody (IRS012) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900808). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.