CD31 Mouse Monoclonal Antibody
cat.: IRS023
| Product Type: | Mouse monoclonal IgG2a, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | mIHC |
| Clonality: | Monoclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 83 kDa |
| Isotype: | IgG2a |
| Immunogen: | Synthetic peptide (KLH-coupled) within C-terminal residues of human CD31. |
| Positive control: | Human tonsil tissue, human gastric cancer tissue, human pancreas tissue, human lung squamous cell carcinoma tissue, human bone tissue. |
| Subcellular location: | Cell junction. Cell membrane. Membrane. |
| Recommended Dilutions:
mIHC |
1:100 |
| Uniprot #: | SwissProt: P16284 Human |
| Alternative names: | Adhesion molecule CD31 CD31 antigen CD31 EndoCAM EndoCAM FLJ34100 FLJ58394 GPIIA GPIIA' PECA1 PECA1_HUMAN Pecam 1 PECAM 1 CD31 EndoCAM PECAM PECAM-1 Pecam1 Platelet and endothelial cell adhesion molecule 1 Platelet endothelial cell adhesion molecule Platelet/endothelial cell adhesion molecule 1 |
Images
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Fig1: mIHC analysis of human tonsil tissue (Formalin/PFA-fixed paraffin-embedded sections) with Ki67 (IRS036), CD23 (IRS021), CD31 (IRS023) and PD-1 antibody at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900802). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner. |
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Fig2: mIHC analysis of human gastric cancer tissue (Formalin/PFA-fixed paraffin-embedded sections) with CD11b (IRS014), CD31 (IRS023), aSMA (IRS012), panCK (IRS010) and CD3 (IRS022) antibody at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900802). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner. |
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Fig3: mIHC analysis of human pancreas tissue (Formalin/PFA-fixed paraffin-embedded sections) with CHGA, CD31 (IRS023), CK19 (IRS011) and Somatostatin 28 antibody at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900802). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner. |
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Fig4: mIHC analysis of human lung squamous cell carcinoma tissue (Formalin/PFA-fixed paraffin-embedded sections) with Mouse anti-CD31 antibody (IRS023) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900809). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner. |
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Fig5: mIHC analysis of human bone tissue (Formalin/PFA-fixed paraffin-embedded sections) with Mouse anti-CD31 antibody (IRS023) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900809). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.