Mannose Receptor(CD206) Recombinant Rabbit Monoclonal Antibody
cat.: IRS049
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Mouse
Applications: mIHC
Clonality: Monoclonal
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 165 kDa
Isotype: IgG
Immunogen: Recombinant protein within mouse Mannose Receptor(CD206) aa 1-1,409.
Positive control: Mouse osteosarcoma tissue, mouse spleen tissue, mouse liver tissue.
Subcellular location: Endosome membrane, Cell membrane.
Recommended Dilutions:
  mIHC
1:100
Uniprot #: SwissProt: Q61830 Mouse
Alternative names: bA541I19.1 C type lectin domain family 13 member D C-type lectin domain family 13 member D CD 206 CD206 CD206 antigen CLEC13D CLEC13DL Macrophage mannose receptor 1 Macrophage mannose receptor 1 like protein 1 Macrophage mannose receptor Mannose receptor C type 1 Mannose receptor C type 1 like 1 MMR MRC 1 MRC1 MRC1_HUMAN MRC1L1 OTTHUMP00000045206
Images
IRS049_1.jpg Fig1: mIHC analysis of mouse osteosarcoma subcutaneous tumor tissue (Formalin/PFA-fixed paraffin-embedded sections) with CD8, F4/80 (IRS052), CD4 (IRS051), CD206 (IRS049) and CD11c antibody at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900802). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
IRS049_2.jpg Fig2: mIHC analysis of mouse spleen tissue (Formalin/PFA-fixed paraffin-embedded sections) with F4/80 (IRS052), B220, CD206 (IRS049), CD4 (IRS051) and CD8 antibody at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900802). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
IRS049_3.jpg Fig3: mIHC analysis of mouse liver tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-Mannose Receptor(CD206) antibody (IRS049) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900808). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
IRS049_4.jpg Fig4: mIHC analysis of mouse osteosarcoma tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-Mannose Receptor(CD206) antibody (IRS049) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900808). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
IRS049_5.jpg Fig5: mIHC analysis of mouse spleen tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-Mannose Receptor(CD206) antibody (IRS049) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900808). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
IRS049_6.jpg Fig6: mIHC analysis of mouse liver tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-Mannose Receptor(CD206) antibody (IRS049) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900808). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
IRS049_7.jpg Fig7: mIHC analysis of mouse osteosarcoma subcutaneous tumor tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-Mannose Receptor(CD206) antibody (IRS049) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900808). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.