F4/80 Recombinant Rabbit Monoclonal Antibody
cat.: IRS052
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Mouse
Applications: mIHC
Clonality: Monoclonal
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 102 kDa
Isotype: IgG
Immunogen: Recombinant protein within mouse F4/80 aa 1-650 / 931.
Positive control: Mouse osteosarcoma subcutaneous tumor tissue, mouse spleen tissue, mouse liver tissue, mouse pancreas tissue.
Subcellular location: Cell membrane.
Recommended Dilutions:
  mIHC
1:100
Uniprot #: SwissProt: Q61549 Mouse
Alternative names: ADGRE1 Adhesion G protein coupled receptor E1 Adhesion G protein-coupled receptor E1 AGRE1_HUMAN Cell surface glycoprotein EMR1 Cell surface glycoprotein F4/80 DD7A5 7 Egf like module containing mucin like hormone receptor like 1 Egf like module containing mucin like hormone receptor like sequence 1 EGF like module receptor 1 EGF TM7 EGF-like module receptor 1 EGF-like module-containing mucin-like hormone receptor-like 1 EGFTM7 EMR 1 EMR1 EMR1 hormone receptor Gpf480 Ly71 Lymphocyte antigen 71 TM7LN3
Images
IRS052_1.jpg Fig1: mIHC analysis of mouse osteosarcoma subcutaneous tumor tissue (Formalin/PFA-fixed paraffin-embedded sections) with F4/80 (IRS052), Ki67 (IRS057), CD8 and CD11c antibody at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900802). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
IRS052_2.jpg Fig2: mIHC analysis of mouse osteosarcoma subcutaneous tumor tissue (Formalin/PFA-fixed paraffin-embedded sections) with CD8, F4/80 (IRS052), CD4 (IRS051), CD206 (IRS049) and CD11c antibody at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900802). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
IRS052_3.jpg Fig3: mIHC analysis of mouse spleen tissue (Formalin/PFA-fixed paraffin-embedded sections) with PD-L1 (IRS059), F4/80 (IRS052), FOXP3 (IRS053), CD11c and CD4 (IRS051) antibody at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900802). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
IRS052_4.jpg Fig4: mIHC analysis of mouse spleen tissue (Formalin/PFA-fixed paraffin-embedded sections) with F4/80 (IRS052), B220, CD206 (IRS049), CD4 (IRS051) and CD8 antibody at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900802). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
IRS052_5.jpg Fig5: mIHC analysis of mouse spleen tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-F4/80 antibody (IRS052) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900808). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
IRS052_6.jpg Fig6: mIHC analysis of mouse liver tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-F4/80 antibody (IRS052) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900808). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
IRS052_7.jpg Fig7: mIHC analysis of mouse osteosarcoma subcutaneous tumor tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-F4/80 antibody (IRS052) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900808). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
IRS052_8.jpg Fig8: mIHC analysis of mouse pancreas tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-F4/80 antibody (IRS052) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900808). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.