Granzyme B Rabbit Polyclonal Antibody
cat.: IRS054
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Mouse
Applications: mIHC
Clonality: Polyclonal
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 28 kDa
Isotype: IgG
Immunogen: Recombinant protein within human Granzyme B aa 50-247.
Positive control: Mouse osteosarcoma tissue, mouse spleen tissue.
Subcellular location: Secreted.
Recommended Dilutions:
  mIHC

1:100
Uniprot #: SwissProt: P04187 Mouse
Alternative names: C11 Cathepsin G like 1 Cathepsin G-like 1 CCPI CGL 1 CGL1 CSP B CSPB CTLA 1 CTLA-1 CTLA1 CTSGL1 Cytotoxic serine protease B Cytotoxic T lymphocyte associated serine esterase 1 Cytotoxic T lymphocyte proteinase 2 Cytotoxic T-lymphocyte proteinase 2 Fragmentin 2 Fragmentin-2 GRAB_HUMAN Granzyme 2 Granzyme B (granzyme 2, cytotoxic T lymphocyte associated serine esterase 1) Granzyme B Granzyme-2 GranzymeB GRB Gzmb Hlp Human lymphocyte protein Lymphocyte protease Protease, serine, B SECT T cell serine protease 1 3E T cell serine protease 1-3E T-cell serine protease 1-3E
Images
IRS054_1.jpg Fig1: mIHC analysis of mouse osteosarcoma subcutaneous tumor tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-Granzyme B antibody (IRS054) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900808). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
IRS054_2.jpg Fig2: mIHC analysis of mouse spleen tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-Granzyme B antibody (IRS054) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900808). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.