RAGE Recombinant Rabbit Monoclonal Antibody
cat.: IRS073
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Mouse
Applications: mIHC
Clonality: Monoclonal
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 43 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human RAGE aa 350-390.
Positive control: Mouse lung tissue.
Subcellular location: Cell membrane, Secreted.
Recommended Dilutions:
  mIHC

1:100
Uniprot #: SwissProt: Q62151 Mouse
Alternative names: Advanced glycosylation end product-specific receptor Ager MGC2235 RAGE_HUMAN Receptor for advanced glycosylation end products
Images
IRS073_1.jpg Fig1: mIHC analysis of mouse lung tissue (Formalin/PFA-fixed paraffin-embedded sections) with TTF1 (IRS063), RAGE (IRS073), CD20, CD4 (IRS051) and aSMA (IRS048) antibody at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900802). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
IRS073_2.jpg Fig2: mIHC analysis of mouse lung tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-RAGE antibody (IRS073) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900808). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
IRS073_3.jpg Fig3: mIHC analysis of mouse lung tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-RAGE antibody (IRS073) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900808). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.