CD8 Rabbit Polyclonal Antibody
cat.: IRS083
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Mouse
Applications: mIHC
Clonality: Polyclonal
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Purification: Affinity purification
Molecular weight: Predicted band size: 27 kDa
Isotype: IgG
Immunogen: Recombinant protein corresponding to Mouse CD8 alpha
Positive control: Mouse spleen tissue, mouse liver tissue.
Subcellular location: Cell membrane.
Recommended Dilutions:
  mIHC

1:100
Uniprot #: SwissProt: P01731 Mouse
Alternative names: alpha polypeptide (p32) CD_antigen=CD8a CD8 CD8 antigen alpha polypeptide CD8 antigen alpha polypeptide (p32) CD8 antigen, alpha polypeptide (p32) CD8a CD8A antigen CD8A molecule CD8A_HUMAN Leu2 Leu2 T lymphocyte antigen Ly 2 Ly 35 Ly B Ly2 Ly3 Ly35 LyB Lyt 2.1 lymphocyte differentiation antigen (AA at 100) LYT3 MAL OKT8 T cell antigen OTTHUMP00000160760 OTTHUMP00000160764 OTTHUMP00000203528 OTTHUMP00000203721 p32 T cell antigen Leu2 T cell co receptor T lymphocyte differentiation antigen T8/Leu 2 T-cell surface glycoprotein CD8 alpha chain T-cell surface glycoprotein Lyt 2 T-lymphocyte differentiation antigen T8/Leu-2 T8 T cell antigen T8/Leu-2 T-lymphocyte differentiation antigen
Images
IRS083_1.jpg Fig1: mIHC analysis of mouse spleen tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-CD8 antibody (IRS083) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900808). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
IRS083_2.jpg Fig2: mIHC analysis of mouse liver tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-CD8 antibody (IRS083) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900808). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.