Estrogen Receptor alpha Recombinant Monoclonal Antibody
cat.: IRS142RB
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: mIHC
Clonality: Monoclonal
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 66 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human Estrogen Receptor alpha aa 300-595 / 595.
Positive control: Human breast tissue.
Subcellular location: Cell membrane, Nucleus, Cytoplasm.
Recommended Dilutions:
  mIHC

1:100
Uniprot #: SwissProt: P03372 Human
Alternative names: 7*/654 isoform 7*/819 2 isoform 7*/822 isoform 8*/901 isoform 8*/941 isoform DKFZp686N23123 ER alpha ER ER-alpha Era ESR ESR1 ESR1_HUMAN ESRA Estradiol receptor Estrogen nuclear receptor alpha Estrogen receptor 1 Estrogen receptor alpha 3*,4,5,6,7*/822 isoform Estrogen receptor alpha Estrogen receptor alpha delta 3*,4,5,6,7*,8*/941 isoform Estrogen receptor alpha delta 3*,4,5,6,7*/819 2 isoform Estrogen receptor alpha delta 4 +49 isoform Estrogen receptor alpha delta 4*,5,6,7*/654 isoform Estrogen receptor alpha delta 4*,5,6,7,8*/901 isoform Estrogen receptor alpha E1 E2 1 2 Estrogen receptor alpha E1 N2 E2 1 2 Estrogen receptor ESTRR NR3A1 Nuclear receptor subfamily 3 group A member 1
Images
IRS142RB_1.jpg Fig1: mIHC analysis of human breast tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-Estrogen Receptor alpha antibody (IRS142RB) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900808). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.