| Product Type: | Recombinant Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | mIHC |
| Clonality: | Monoclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 29 kDa |
| Isotype: | IgG1 |
| Immunogen: | Synthetic peptide within Human HLA-DR aa 25-66/254. |
| Positive control: | Human tonsil tissue. |
| Subcellular location: | Cell membrane. Endoplasmic reticulum membrane. |
| Recommended Dilutions:
mIHC |
1:100 |
| Uniprot #: | SwissProt: P01903 Human |
| Alternative names: | DR alpha chain DR alpha chain precursor DRA_HUMAN DRB1 DRB4 Histocompatibility antigen HLA DR alpha HLA class II histocompatibility antigen HLA class II histocompatibility antigen DR alpha chain HLA DR1B HLA DR3B HLA DRA HLA DRA1 HLA DRB1 HLA DRB3 HLA DRB4 HLA DRB5 HLA-DRA HLADR4B HLADRA1 HLADRB Major histocompatibility complex class II DR alpha Major histocompatibility complex class II DR beta 1 Major histocompatibility complex class II DR beta 3 Major histocompatibility complex class II DR beta 4 Major histocompatibility complex class II DR beta 5 MGC117330 MHC cell surface glycoprotein MHC class II antigen DRA MHC II MLRW |
|
Fig1: mIHC analysis of human tonsil tissue (Formalin/PFA-fixed paraffin-embedded sections) with Mouse anti-HLA-DR antibody (IRS154MS) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900809). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner. |
|
Fig2: mIHC analysis of human tonsil tissue (Formalin/PFA-fixed paraffin-embedded sections) with Mouse anti-HLA-DR antibody (IRS154MS) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900809). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner. |
|
Fig3: mIHC analysis of human tonsil tissue (Formalin/PFA-fixed paraffin-embedded sections) with Mouse anti-HLA-DR antibody (IRS154MS) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900809). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner. |