| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | mIHC |
| Clonality: | Monoclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 53 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within mouse HNF-4-alpha aa 1-250/474. |
| Positive control: | Human liver tissue. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
mIHC |
1:100 |
| Uniprot #: | SwissProt: P41235 Human |
| Alternative names: | FLJ39654 FRTS4 Hepatic nuclear factor 4 alpha Hepatocyte nuclear factor 4 alpha Hepatocyte nuclear factor 4 Hepatocyte nuclear factor 4-alpha HNF 4 alpha HNF 4 HNF-4-alpha HNF4 HNF4A HNF4A_HUMAN HNF4a7 HNF4a8 HNF4a9 Hnf4alpha HNF4alpha10/11/12 MODY 1 MODY MODY1 NR2A1 NR2A21 Nuclear receptor subfamily 2 group A member 1 OTTHUMP00000031060 OTTHUMP00000031062 TCF 14 TCF TCF-14 TCF14 Tcf4 Transcription factor 14, hepatic nuclear factor Transcription factor 14 Transcription factor HNF 4 Transcription factor HNF-4 Transcription factor HNF4 |
|
Fig1: mIHC analysis of human liver tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-HNF-4-alpha antibody (IRS155RB) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900808). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner. |