| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | mIHC |
| Clonality: | Monoclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 121 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human SLC12A1 / NKCC2 aa 593-813 / 1,099. |
| Positive control: | Human kidney tissue. |
| Subcellular location: | Cell membrane |
| Recommended Dilutions:
mIHC |
1:100 |
| Uniprot #: | SwissProt: Q13621 Human |
| Alternative names: | BSC1 Bumetanide sensitive sodium 3 Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 2 Kidney specific Na K Cl symporter Kidney-specific Na-K-Cl symporter MGC48843 Na K 2Cl cotransporter NKCC2 potassiumchloride cotransporter 2 S12A1_HUMAN Slc12a1 sodium potassium chloride cotransporter 2 solute carrier family 12 (sodium/potassium/chloride transporters) Solute carrier family 12 member 1 |
|
Fig1: mIHC analysis of human kidney tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-SLC12A1 / NKCC2 antibody (IRS181RB) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900808). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner. |