Cytokeratin 5 Recombinant Rabbit Monoclonal Antibody
cat.: IRS249RB
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: mIHC
Clonality: Monoclonal
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage buffer: 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 62 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human Cytokeratin 5 aa 70-110.
Positive control: Human oral squamous cell carcinoma tissue, human pancreatic cancer tissue.
Subcellular location: Cytoplasm.
Recommended Dilutions:
  mIHC

1:100
Uniprot #: SwissProt: P13647 Human
Alternative names: 58 kDa cytokeratin CK-5 CK5 Cytokeratin-5 Cytokeratin5 DDD DDD1 EBS2 epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne types K2C5_HUMAN K5 keratin 5 (epidermolysis bullosa simplex, Dowling-Meara/Kobner/Weber-Cockayne types) Keratin 5 Keratin keratin complex 2, basic, gene 5 keratin, type II cytoskeletal 5 Keratin-5 Keratin5 KRT 5 Krt5 KRT5A type II cytoskeletal 5 Type-II keratin Kb5
Images
IRS249RB_1.jpg Fig1: mIHC analysis of human oral squamous cell carcinoma tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-Cytokeratin 5 antibody (IRS249RB) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900808). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
IRS249RB_2.jpg Fig2: mIHC analysis of human pancreatic cancer tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-Cytokeratin 5 antibody (IRS249RB) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900808). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.