| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | mIHC |
| Clonality: | Monoclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 35 kDa |
| Isotype: | IgG |
| Positive control: | Human colon tissue. |
| Subcellular location: | Lateral cell membrane, Cell junction. |
| Recommended Dilutions:
mIHC |
1:100 |
| Uniprot #: | SwissProt: P16422 Human |
| Alternative names: | 17 1A 323/A3 Adenocarcinoma associated antigen Adenocarcinoma-associated antigen Antigen identified by monoclonal AUA1 AUA1 CD326 CD326 antigen Cell surface glycoprotein Trop 1 Cell surface glycoprotein Trop 2 Cell surface glycoprotein Trop-1 CO 17A CO17 1A CO17A DIAR5 EGP 2 EGP EGP2 EGP314 EGP40 Ep CAM Ep-CAM EPCAM EPCAM_HUMAN EpCAM1 Epithelial cell adhesion molecule Epithelial Cell Adhesion Molecule Intracellular Domain (EpCAM-ICD) Epithelial cell surface antigen Epithelial cellular adhesion molecule Epithelial glycoprotein 1 Epithelial glycoprotein 314 Epithelial glycoprotein ESA GA733 1 GA733 2 GA733-2 gastrointestinal tumor-associated antigen 2, 35-KD glycoprotein gp4 hEGP 2 hEGP314 HNPCC8 Human epithelial glycoprotein 2 KS 1/4 antigen KS1/4 KSA Ly74 Lymphocyte antigen 74 M1S 1 M1S2 M4S1 Major gastrointestinal tumor associated protein GA733 2 Major gastrointestinal tumor-associa...... |
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Fig1: mIHC analysis of human colon tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-EpCAM antibody (IRS254RB) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900808). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner. |