Autophagy Antibody Sampler Kit
cat.: K2002
| Product Type: | Antibody Sampler Kit |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Uniprot #: | SwissProt: Q9GZQ8 Human | Q9CQV6 Mouse | Q62625 Rat | Q9H1Y0 Human | Q99J83 Mouse | Q3MQ06 Rat | O94817 Human | Q676U5 Human | Q8C0J2 Mouse | O95352 Human | Q641Y5 Rat | Q9NT62 Human | Q9CPX6 Mouse | Q6AZ50 Rat | Q14457 Human | O88597 Mouse | Q91XJ1 Rat |
| Alternative names: | ATG8F Autophagy-related protein LC3 B Autophagy-related ubiquitin-like modifier LC3 B LC3B LC3II MAP1 light chain 3 like protein 2 MAP1 light chain 3-like protein 2 MAP1A/1BLC3 MAP1A/MAP1B LC3 B MAP1A/MAP1B light chain 3 B MAP1ALC3 MAP1LC3B a Map1lc3b Microtubule associated protein 1 light chain 3 beta Microtubule-associated protein 1 light chain 3 beta Microtubule-associated proteins 1A/1B light chain 3B MLP3B_HUMAN |
Images
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Fig1:
Western blot analysis of LC3B on different lysates with Rabbit anti-LC3B antibody (ET1701-65) at 1/2,000 dilution and competitor's antibody at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 50μM Chloroquine for 18 hours cell lysate Lane 3: C2C12 cell lysate Lane 4: C2C12 treated with 50μM Chloroquine for 18 hours cell lysate Lane 5: C6 cell lysate Lane 6: C6 treated with 50μM Chloroquine for 18 hours cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 14/16 kDa Observed band size: 14/16 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1701-65) at 1/2,000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of ATG5 on different lysates with Rabbit anti-ATG5 antibody (ET1611-38) at 1/5,000 dilution and competitor's antibody at 1/1,000 dilution. Lane 1: NIH/3T3 cell lysate (15 µg/Lane) Lane 2: C2C12 cell lysate (15 µg/Lane) Lane 3: Neuro-2a cell lysate (15 µg/Lane) Lane 4: PC-12 cell lysate (15 µg/Lane) Lane 5: Mouse brain tissue lysate (15 µg/Lane) Lane 6: Rat brain tissue lysate (15 µg/Lane) Predicted band size: 32 kDa Observed band size: 55 kDa Exposure time: 35 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-38) at 1/5,000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.