Cell Cycle Phase Determination Antibody Kit
cat.: K2004
| Product Type: | Antibody Sampler Kit |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Uniprot #: | SwissProt: O75496 Human | O88513 Mouse | Q9H211 Human | P04183 Human | P04184 Mouse | P68431 Human | P84243 Human | Q16695 Human | Q6NXT2 Human | Q71DI3 Human | P84244 Mouse | P84245 Rat | P20248 Human | P51943 Mouse | P14635 Human | P24864 Human | Q61457 Mouse Entrez Gene: 291137 Rat Unigene: 13094 Rat |
| Alternative names: | DNA replication inhibitor Gem GEMI_HUMAN Geminin Geminin DNA replication inhibitor GMNN OTTHUMP00000039393 RP3 369A17.3 |
Images
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Fig1:
Western blot analysis of Phospho-Histone H3 (S10) on different lysates with Rabbit anti-Phospho-Histone H3 (S10) antibody (ET1601-30) at 1/5,000 dilution. Lane 1: HeLa whole cell lysate (20 µg/Lane) Lane 2: HeLa treated with 50nM Calyculin A for 30 minutes whole cell lysate (20 µg/Lane) Lane 3: NIH/3T3 whole cell lysate (20 µg/Lane) Lane 4: NIH/3T3 treated with 100nM Calyculin A for 30 minutes whole cell lysate (20 µg/Lane) Lane 5: C6 whole cell lysate (20 µg/Lane) Lane 6: C6 treated with 100nM Calyculin A for 30 minutes whole cell lysate (20 µg/Lane) Predicted band size: 15 kDa Observed band size: 15 kDa Exposure time: 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1601-30) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of Cyclin B1 on different lysates with Rabbit anti-Cyclin B1 antibody (ET1608-27) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: Jurkat cell lysate Lane 3: HepG2 cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 48 kDa Observed band size: 55 kDa Exposure time: 3 minutes 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1608-27) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.