Cytokeratin 17 Mouse Monoclonal Antibody [83-13]
cat.: M0407-13
| Product Type: | Mouse monoclonal IgG2a, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | 83-13 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 48 kDa |
| Isotype: | IgG2a |
| Immunogen: | Synthetic peptide within Human Cytokeratin 17 aa 383-432 / 432. |
| Positive control: | Hela cell lysate, Hela, PC-3M, SK-Br-3, human breast carcinoma tissue, mouse prostate tissue. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:500-1:2,000 1:100 1:100-1:200 |
| Uniprot #: | SwissProt: Q04695 Human | Q9QWL7 Mouse | Q6IFU8 Rat |
| Alternative names: | 39.1 CK 17 CK17 CK-17 Cytokeratin-17 K17 K1C17_HUMAN Keratin 17 keratin 17 epitope S1 keratin 17 epitope S2 keratin 17 epitope S4 Keratin 17, type I Keratin Keratin type I cytoskeletal 17 keratin, type i cytoskeletal 17 [version 1] Keratin-17 KRT17 PC PC2 PCHC1 type I cytoskeletal 17 |
Images
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Fig1: ICC staining Cytokeratin 17 in Hela cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with Cytokeratin 17 monoclonal antibody at a dilution of 1/100 for 1 hour at room temperature, washed with PBS. Alexa Fluorc™555 Goat anti-Mouse IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue). |
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Fig2: ICC staining Cytokeratin 17 in PC-3M cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with Cytokeratin 17 monoclonal antibody at a dilution of 1/100 for 1 hour at room temperature, washed with PBS. Alexa Fluorc™555 Goat anti-Mouse IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue). |
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Fig3: ICC staining Cytokeratin 17 in SK-Br-3 cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with Cytokeratin 17 monoclonal antibody at a dilution of 1/100 for 1 hour at room temperature, washed with PBS. Alexa Fluorc™555 Goat anti-Mouse IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue). |
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Fig4: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Cytokeratin 17 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (M0407-13) at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX. |
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Fig5: Immunohistochemical analysis of paraffin-embedded mouse prostate tissue using anti-Cytokeratin 17 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (M0407-13) at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX. |
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Fig6:
Western blot analysis of Cytokeratin 17 on different lysates with Mouse anti-Cytokeratin 17 antibody (M0407-13) at 1/1,000 dilution. Lane 1: HeLa (Human cervical cancer cell) cell lysate Lane 2: HEK-293 (Human embryonic Kidney cell) cell lysate Lane 3: SiHa (Human cervical squamous cell) cell lysate Lane 4: PC-3M (Human prostatic carcinoma cell) cell lysate Exposure time: 25 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: M0407-13, 1/1,000 in 5% NFDM/TBST, overnight at 4 ℃ Predicted band size: 48 kDa Observed band size: 48 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.