14-3-3 alpha+beta Mouse Monoclonal Antibody [9-26]
cat.: M0407-14
| Product Type: | Mouse monoclonal IgG2a, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Zebrafish, Rat |
| Applications: | WB, IF-Cell, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | 9-26 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 28 kDa |
| Isotype: | IgG2a |
| Immunogen: | Recombinant protein within Human 14-3-3 b / a aa 1-246 / 246. |
| Positive control: | A549 cell lysates, mouse liver tissue lysates, hybrid fish (crucian-carp) brain tissue lysates, 293T, human breast carcinoma tissue, Zebrafish tissue. |
| Subcellular location: | Cytoplasm, Membrane, Vacuole. |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:1,000-1:5,000 1:50-1:100 1:200 |
| Uniprot #: | SwissProt: P31946 Human |
| Alternative names: | 14 3 3 alpha 14 3 3 protein beta/alpha 14-3-3 protein beta/alpha 1433B_HUMAN Brain protein 14 3 3 beta isoform GW128 HS 1 KCIP-1 KCIP1 N-terminally processed Protein 1054 Protein kinase C inhibitor protein 1 YWHAA YWHAB |
Images
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Fig1:
Western blot analysis of 14-3-3 alpha+beta on A549 cell lysates with Mouse anti-14-3-3 alpha+beta antibody (M0407-14) at 1/5,000 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 28 kDa Observed band size: 28 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M0407-14) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature. |
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Fig2: Western blot analysis on mouse liver tissue lysates using anti-14-3-3b/a Mouse mAb (Cat. # M0407-14). |
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Fig3: Western blot analysis of 14-3-3b/a on hybrid fish (crucian-carp) brain tissue lysate using anti-14-3-3b/a antibody at 1/500 dilution. |
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Fig4: Immunocytochemistry analysis of 293T cells labeling 14-3-3 alpha+beta with Mouse anti-14-3-3 alpha+beta antibody (M0407-14) at 1/50 dilution. |
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Fig5: Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using anti-14-3-3b/a Mouse mAb (Cat. # M0407-14). |
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Fig6: Immunohistochemical analysis of paraffin-embedded Zebrafish tissue using anti-14-3-3b/a antibody. Counter stained with hematoxylin. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.