Product Type: | Mouse monoclonal IgG2a, primary antibodies |
---|---|
Species reactivity: | Human |
Applications: | WB, ELISA |
Clonality: | Monoclonal |
Clone number: | 54-76 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 2ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | 50 kDa |
Isotype: | IgG2a |
Immunogen: | Native protein. |
Positive control: | Human IgG protein lysates. |
Subcellular location: | Cell membrane, Immunoglobulin, Membrane, Secreted. |
Recommended Dilutions:
WB ELISA |
1:5,000 1:5,000 |
Uniprot #: | SwissProt: P01857 Human | P01859 Human | P01860 Human | P01861 Human |
Alternative names: | FLJ39988 FLJ40036 FLJ40253 FLJ40587 FLJ40789 FLJ40834 G1m marker G2m marker G3m marker G4m marker HDC Heavy chain disease protein Human immunglobulin G Ig gamma 1 chain C region Ig gamma 2 chain C region Ig gamma 3 chain C region Ig gamma 4 chain C region IgG heavy chain locus IGHG 1 IGHG 2 IGHG 3 IGHG 4 IGHG1 IGHG2 IGHG3 IGHG4 Immunoglobulin Gm1 Immunoglobulin Gm3 Immunoglobulin Gm4 immunoglobulin heavy constant gamma 1 (G1m marker) Immunoglobulin heavy constant gamma 1 immunoglobulin heavy constant gamma 2 (G2m marker) Immunoglobulin heavy constant gamma 2 immunoglobulin heavy constant gamma 3 (G3m marker) Immunoglobulin heavy constant gamma 3 immunoglobulin heavy constant gamma 4 (G4m marker) Immunoglobulin heavy constant gamma 4 MGC117419 MGC45809 |
Fig1: Western blot analysis of Human IgG heavy chain on human IgG protein lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (M0806-1, 1/5000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature. |