TdT Mouse Monoclonal Antibody [B4-F5]
cat.: M0807-13
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | B4-F5 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 58kDa |
| Isotype: | IgG1 |
| Immunogen: | Synthetic peptide (KLH-coupled) corresponding to near C-terminal residues of human TdT. |
| Positive control: | Jurkat, Hela |
| Subcellular location: | Cell nucleus |
| Recommended Dilutions:
WB |
1:2,000 |
| Uniprot #: | SwissProt: P04053 Human |
| Alternative names: | Deoxynucleotidyltransferase terminal DNA nucleotidylexotransferase DNTT Nucleosidetriphosphate DNA deoxynucleotidylexotransferase TDT TDT_HUMAN Terminal addition enzyme Terminal deoxynucleotidyltransferase Terminal deoxyribonucleotidyltransferase Terminal transferase |
Images
|
Fig1:
Western blot analysis of TdT on different lysates with Rabbit anti-TdT antibody (M0807-13) at 1/2,000 dilution. Lane 1: Jurkat (Human T lymphocyte cell) cell lysate Lane 2: HL-60 (Human myeloid leukemia cell) cell lysate Lane 3: Mouse liver tissue lysate Lysates/proteins at 20 µg/Lane. Exposure time: 1 minutes 50seconds; ECL: K1802 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: M0807-13, 1/2,000 in primary antibody dilution buffer 5% NFDM/TBST, overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 58 kDa Observed band size: 58 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.