Rabbit IgG kappa Light Chain Mouse Monoclonal Antibody [1-14]
cat.: M0809-1
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Rabbit |
| Applications: | WB, ELISA, IP |
| Clonality: | Monoclonal |
| Clone number: | 1-14 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 25 kDa |
| Isotype: | IgG1 |
| Immunogen: | Purified Rabbit IgG κ Light Chain. |
| Positive control: | Rabbit IgG |
| Subcellular location: | serum/plasma |
| Recommended Dilutions:
IP WB ELISA |
1:500-1,000 1:500-1,000 1:1,000-2,000 |
| Alternative names: | IgG κ IgGκ |
Images
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Fig1:
Western blot analysis of Rabbit IgG kappa Light Chain on different lysates with Rabbit anti-Rabbit IgG kappa Light Chain antibody (M0809-1) at 1/1,000 dilution. Lane 1: Rabbit IgG lysate Lane 2: Mouse IgG lysate Lane 3: Human IgG lysate Lysates/proteins at 10 ng/Lane. Exposure time: 30 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: M0809-1, 1/1,000 in 5% NFDM/TBST, overnight at 4 ℃ Secondary antibody: Goat Anti-Mouse IgG-HRP (HA1006), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 25 kDa Observed band size: 25 kDa |
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Fig2: Indirect ELISA analysis of Rabbit IgG kappa Light Chain was performed by coating wells of a 96-well plate with 50 µL per well of Rabbit IgG / Mouse IgG / Human IgG diluted in carbonate/bicarbonate buffer, at a concentration of 1 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 1%BSA blocking buffer for 1 hour at 37℃, and incubated with 50 µL per well of Rabbit IgG kappa Light Chain monoclonal antibody serial diluted starting from a concentration of 1µg/mL for 45 minutes at 37℃. The plate was washed and incubated with 50 µL per well of an HRP-conjugated goat anti-mouse IgG secondary antibody at a dilution of 1/80,000 for 30 minutes at 37℃. Detection was performed using an Ultra TMB Substrate for 10 minutes at 37℃ in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.