Apolipoprotein A1 Mouse Monoclonal Antibody
cat.: M0809-13
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, IHC-P |
| Clonality: | Monoclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 31 kDa |
| Isotype: | IgG1 |
| Immunogen: | Synthetic peptide within human Apolipoprotein A1 aa 19-68. |
| Positive control: | Human plasma lysates, human liver tissue. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:500-1:1,000 1:50-1:200 1:50-1:200 |
| Uniprot #: | SwissProt: P02647 Human |
| Alternative names: | Apo-AI ApoA I ApoA-I APOA1 APOA1_HUMAN Apolipoprotein A-I(1-242) Apolipoprotein A1 Apolipoprotein AI Apolipoprotein of high density lipoprotein ApolipoproteinAI Brp14 high density lipoprotein uptake Ltw1 Lvtw1 MGC117399 Sep1 Sep2 |
Images
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Fig1:
Western blot analysis of Apolipoprotein A1 on human plasma lysates with Mouse anti-Apolipoprotein A1 antibody (M0809-13) at 1/1,000 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 31 kDa Observed band size: 26 kDa Exposure time: 5 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M0809-13) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-Apolipoprotein A1 antibody (M0809-13) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M0809-13) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.