6X His tag Mouse Monoclonal Antibody [8-F0]
cat.: M0812-3
| Product Type: | Mouse monoclonal IgG2a, primary antibodies |
|---|---|
| Species reactivity: | Species independent |
| Applications: | WB, IP |
| Clonality: | Monoclonal |
| Clone number: | 8-F0 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. |
| Concentration: | 2ug/ul |
| Purification: | Protein G affinity purified. |
| Isotype: | IgG2a |
| Immunogen: | Synthetic peptide immune sequence is CHHHHHHHH. |
| Positive control: | C-terminal His-tagged recombinant protein. |
| Recommended Dilutions:
WB IP |
1:5,000 2-5 µg/ml. |
| Alternative names: | 6 His epitope tag Hexa His tag HHHHHH epitope tag HHHHHH tag His tag Polyhistidine Tag |
Images
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Fig1:
His tag was immunoprecipitated in 2µg C terminal His Tag fusion protein lysate with M0812-3 at 2 µg/20 µl agarose. Western blot was performed from the immunoprecipitate using R1207-2 at 1/500 dilution. Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 60 mins at room temperature. Lane 1: His Tag fusion protein lysate (input). Lane 2: M0812-3 IP in His Tag fusion protein lysate. Lane 3: Mouse IgG instead of M0812-3 in His Tag fusion protein lysate. Blocking/Dilution buffer: 5% NFDM/TBST |
|
Fig2:
Western blot analysis of 6X His tag on His-tagged recombinant protein with Mouse anti-6X His tag antibody (M0812-3) at 1/5,000 dilution. Lane 1: N-terminal His-tagged recombinant protein Lane 2: C-terminal His-tagged recombinant protein Lysates/proteins at 50 ng/Lane. Exposure time: 100 seconds; ECL: K1802 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: M0812-3, 1/5,000 in 5% NFDM/TBST, overnight at 4 ℃ Secondary antibody: Goat anti-Mouse IgG-HRP (HA1006), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 30 kDa Observed band size: 30 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.