| Product Type: | Mouse monoclonal IgG2b, primary antibodies |
|---|---|
| Species reactivity: | Goat |
| Applications: | WB, ELISA, IP |
| Clonality: | Monoclonal |
| Clone number: | B8-C4-G4 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein G affinity purified. |
| Molecular weight: | 25 kDa |
| Isotype: | IgG2b |
| Immunogen: | Native protein. |
| Positive control: | Goat IgG protein lysates. |
| Recommended Dilutions:
WB ELISA IP |
1:1,000-1:2,000 1:2,000-1:5,000 1:100-1:500 |
|
Fig1: Western blot analysis of Goat IgG Light chain on Goat IgG protein lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (M0910-2, 1/1000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature. |