Product Type: | Mouse monoclonal IgG2b, primary antibodies |
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Species reactivity: | Goat |
Applications: | WB, ELISA, IP |
Clonality: | Monoclonal |
Clone number: | B8-C4-G4 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 2ug/ul |
Purification: | Protein G affinity purified. |
Molecular weight: | 25 kDa |
Isotype: | IgG2b |
Immunogen: | Native protein. |
Positive control: | Goat IgG protein lysates. |
Recommended Dilutions:
WB ELISA IP |
1:1,000-1:2,000 1:2,000-1:5,000 1:100-1:500 |
Fig1: Western blot analysis of Goat IgG Light chain on Goat IgG protein lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (M0910-2, 1/1000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature. |