Kidins220 Mouse Monoclonal Antibody [3-B6]
cat.: M0910-4
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Human |
| Applications: | WB, IF-Cell, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | 3-B6 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 197kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein with Human kinase D-interacting substrate 220 aa 1601-1771 / 1,771. |
| Positive control: | K562 cell lysates, F9 cells, mouse brain tissue. |
| Subcellular location: | Cell membrane |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:1,000-1:10,000 1:100 1:50 |
| Uniprot #: | SwissProt: Q9ULH0 Human | E9Q9B7 Mouse |
| Alternative names: | Ankyrin repeat-rich membrane-spanning protein arms KDIS_HUMAN kidins220 Kinase D interacting substance of 220 kDa Kinase D-interacting substrate of 220 kDa rgd 619949 |
Images
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Fig1: Western blot analysis of Kidins220 on K562 cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (M0910-4, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature. |
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Fig2: Western blot analysis on recombinant protein using anti-Kidins220 Mouse mAb (Cat. # M0910-4). |
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Fig3: Immunofluorescent staining of F9 cells using anti-Kidins220 Mouse mAb (Cat. # M0910-4). |
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Fig4: Immunohistochemical analysis of paraffin- embedded mouse brain tissue using anti-Kidins220 Mouse mAb (Cat. # M0910-4). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.