Neuropilin 1 Mouse Monoclonal Antibody [A3-D1]
cat.: M0910-5
| Product Type: | Mouse monoclonal IgM, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IF-Cell, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | A3-D1 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 103 kDa |
| Isotype: | IgM |
| Immunogen: | Recombinant protein with mouse Neuropilin 1 aa 856-923 / 923. |
| Positive control: | Hela, mouse brain tissue, Human kidney tissue, mouse kidney tissue. |
| Subcellular location: | Cell membrane, Cytoplasm, Secreted. |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:1,000-1:10,000 1:100-1:500 1:50-1:400 |
| Uniprot #: | SwissProt: O14786 Human | P97333 Mouse |
| Alternative names: | AI851453 BCTL1 Brain specific transmembrane protein containing 2 CUB and 1 LDL receptor class A domains protein 1 Brain-specific transmembrane protein containing 2 CUB and 1 LDL-receptor class A domains protein 1 BTCL1 C130005O10Rik FLJ41325 Neto1 NETO1_HUMAN Neuropilin and tolloid like 1 Neuropilin and tolloid-like protein 1 RGD1566269 |
Images
|
Fig1: Western blot analysis on recombinant protein using anti-Neuropilin 1 Mouse mAb (Cat. # M0910-5). |
|
Fig2: Immunofluorescent staining of Hela cells using anti-Neuropilin 1 Mouse mAb (Cat. # M0910-5). |
|
Fig3: Immunohistochemical analysis of paraffin- embedded mouse brain tissue using anti-Neuropilin 1 Mouse mAb (Cat. # M0910-5). |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Mouse anti-Neuropilin 1 antibody (M0910-5) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M0910-5) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig5:
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Mouse anti-Neuropilin 1 antibody (M0910-5) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M0910-5) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.