CCDC47 Mouse Monoclonal Antibody [1-B9-5]
cat.: M1004-3
Product Type: Mouse monoclonal IgG1, primary antibodies
Species reactivity: Human, Mouse
Applications: IF-Cell, IHC-P, WB
Clonality: Monoclonal
Clone number: 1-B9-5
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 2ug/ul
Purification: Protein G affinity purified.
Molecular weight: Predicted band size: 56 kDa
Isotype: IgG1
Immunogen: Recombinant protein within Human CCDC47 aa 1-483 / 483.
Positive control: Recombinant protein, F9, MCF-7, human liver tissue, human lung tissue, human testis tissue, human breast tissue, human colon tissue, human stomach tissue.
Subcellular location: Endoplasmic reticulum membrane.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
1:500-1:1,000
1:50-1:200
1:50-1:200
Uniprot #: SwissProt: Q96A33 Human | Q9D024 Mouse
Alternative names: CCD47_HUMAN CCDC 47 CCDC47 Coiled coil domain containing 47 Coiled coil domain containing protein 47 Coiled-coil domain-containing protein 47 GK001 MSTP041
Images
M1004-3_1.jpg Fig1: Western blot analysis of CCDC47 on recombinant protein using anti-CCDC47 antibody at 1/1,000 dilution.
M1004-3_2.png Fig2: Western blot analysis of CCDC47 on Mouse heart tissue lysates with Mouse anti-CCDC47 antibody (M1004-3) at 1/1,000 dilution.

Lysates/proteins at 20 µg/Lane.

Predicted band size: 56 kDa
Observed band size: 56 kDa

Exposure time: 2 minutes;

10% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1004-3) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature.
M1004-3_3.jpg Fig3: ICC staining CCDC47 in F9 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
M1004-3_4.png Fig4: Immunocytochemistry analysis of MCF-7 cells labeling CCDC47 with Mouse anti-CCDC47 antibody (M1004-3) at 1/50 dilution.

Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% BSA for 30 minutes at room temperature. Cells were then incubated with Mouse anti-CCDC47 antibody (M1004-3) at 1/50 dilution in 2% BSA overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
M1004-3_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-CCDC47 antibody. Counter stained with hematoxylin.
M1004-3_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-CCDC47 antibody. Counter stained with hematoxylin.
M1004-3_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded human testis tissue using anti-CCDC47 antibody. Counter stained with hematoxylin.
M1004-3_8.jpg Fig8: Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-CCDC47 antibody. Counter stained with hematoxylin.
M1004-3_9.jpg Fig9: Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-CCDC47 antibody. Counter stained with hematoxylin.
M1004-3_10.jpg Fig10: Immunohistochemical analysis of paraffin-embedded human stomach tissue using anti-CCDC47 antibody. Counter stained with hematoxylin.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.