SCAI Mouse Monoclonal Antibody [7-A4]
cat.: M1004-5
| Product Type: | Mouse monoclonal IgM, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | 7-A4 |
| Form: | Liquid |
| Storage condition: | Store at -20℃. Stable for 12 months from date of receipt. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein L affinity purified. |
| Molecular weight: | Predicted band size: 70 kDa |
| Isotype: | IgM |
| Immunogen: | Recombinant protein within Human SCAI aa 1-606 / 606. |
| Positive control: | Recombinant protein, SHG-44, SH-SY-5Y, PC-12, human tonsil tissue, human lung tissue, human liver tissue, human brain tissue, human heart tissue, human pancreas tissue, human colon tissue |
| Subcellular location: | Membrane. Nucleus. Cytoplasm. |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:1,000-1:2,000 1:50-1:200 1:50-1:400 |
| Uniprot #: | SwissProt: Q8N9R8 Human |
| Alternative names: | C9orf126 FLJ11758 FLJ36664 MGC120525 MGC120526 MGC120528 NET40 Protein SCAI SCAI SCAI_HUMAN Suppressor of cancer cell invasion Suppressor of cancer cell invasion protein |
Images
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Fig1: Western blot analysis of SCAI on Recombinant protein using anti-SCAI antibody at 1/1,000 dilution. |
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Fig2: ICC staining SCAI in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining SCAI in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: ICC staining SCAI in PC-12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-SCAI antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-SCAI antibody. Counter stained with hematoxylin. |
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Fig7: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-SCAI antibody. Counter stained with hematoxylin. |
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Fig8: Immunohistochemical analysis of paraffin-embedded human brain tissue using anti-SCAI antibody. Counter stained with hematoxylin. |
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Fig9: Immunohistochemical analysis of paraffin-embedded human heart tissue using anti-SCAI antibody. Counter stained with hematoxylin. |
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Fig10: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-SCAI antibody. Counter stained with hematoxylin. |
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Fig11:
Immunohistochemical analysis of paraffin-embedded human colon tissue with Mouse anti-SCAI antibody (M1004-5) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1004-5) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.