Golgin subfamily A member 5 Mouse Monoclonal Antibody [11-D7]
cat.: M1004-6
Product Type: Mouse monoclonal IgG2a, primary antibodies
Species reactivity: Human
Applications: WB, IHC-P
Clonality: Monoclonal
Clone number: 11-D7
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 2ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 83 kDa
Isotype: IgG2a
Immunogen: Recombinant protein within Human Golgin subfamily A member 5 aa 371-626 / 731.
Positive control: Hela cell lysate, K562 cell lysate, gastric cancer tissue.
Subcellular location: Golgi apparatus membrane
Recommended Dilutions:
  WB
  IHC-P

1:1,000
1:100
Uniprot #: SwissProt: Q8TBA6 Human
Alternative names: Cell proliferation inducing gene 31 Cell proliferation-inducing gene 31 protein GOGA5_HUMAN GOLGA 5 GOLGA5 Golgi autoantigen golgin subfamily a 5 Golgi integral membrane protein 5 Golgin 84 Golgin A5 Golgin subfamily A member 5 Golgin-84 Golgin84 GOLIM 5 GOLIM5 Protein Ret-II PTC5 Ret fused gene 5 RET fused gene 5 protein Ret II Ret II protein RET-fused gene 5 protein RFG 5 RFG5
Images
M1004-6_1.png Fig1: Western blot analysis of Golgin subfamily A member 5 on different lysates with Mouse anti-Golgin subfamily A member 5 antibody (M1004-6) at 1/1,000 dilution.

Lane 1: Hela cell lysate
Lane 2: K562 cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 83 kDa
Observed band size: 83 kDa

Exposure time: 2 minutes;

6% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1004-6) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature.
M1004-6_2.jpg Fig2: Immunohistochemical analysis of paraffin- embedded gastric cancer tissue using anti-GOLGA5 Mouse mAb (Cat. # M1004-6).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.