Product Type: | Mouse monoclonal IgG2a, primary antibodies |
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Species reactivity: | Human |
Applications: | WB, IHC-P |
Clonality: | Monoclonal |
Clone number: | 11-D7 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 2ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | Predicted band size: 83 kDa |
Isotype: | IgG2a |
Immunogen: | Recombinant protein within Human Golgin subfamily A member 5 aa 371-626 / 731. |
Positive control: | Hela cell lysate, K562 cell lysate, gastric cancer tissue. |
Subcellular location: | Golgi apparatus membrane |
Recommended Dilutions:
WB IHC-P |
1:1,000 1:100 |
Uniprot #: | SwissProt: Q8TBA6 Human |
Alternative names: | Cell proliferation inducing gene 31 Cell proliferation-inducing gene 31 protein GOGA5_HUMAN GOLGA 5 GOLGA5 Golgi autoantigen golgin subfamily a 5 Golgi integral membrane protein 5 Golgin 84 Golgin A5 Golgin subfamily A member 5 Golgin-84 Golgin84 GOLIM 5 GOLIM5 Protein Ret-II PTC5 Ret fused gene 5 RET fused gene 5 protein Ret II Ret II protein RET-fused gene 5 protein RFG 5 RFG5 |
Fig1:
Western blot analysis of Golgin subfamily A member 5 on different lysates with Mouse anti-Golgin subfamily A member 5 antibody (M1004-6) at 1/1,000 dilution. Lane 1: Hela cell lysate Lane 2: K562 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 83 kDa Observed band size: 83 kDa Exposure time: 2 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1004-6) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature. |
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Fig2: Immunohistochemical analysis of paraffin- embedded gastric cancer tissue using anti-GOLGA5 Mouse mAb (Cat. # M1004-6). |