GFP Mouse Monoclonal Antibody [B2-F7-C10]
cat.: M1004-8
| Product Type: | Mouse monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Species independent |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | B2-F7-C10 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein G affinity purified. |
| Molecular weight: | 26 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant full length protein of Aequorea victoria GFP. |
| Positive control: | K562 cell lysates transfected with GFP. |
| Recommended Dilutions:
WB |
1:1,000-1:5,000 |
| Uniprot #: | SwissProt: P42212 AequoreaVictoria |
| Alternative names: | GFP Green fluorescent protein |
Images
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Fig1: Western blot analysis of GFP on K562 cell lysates transfected with GFP. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (M1004-8, 1/1000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.