M1005-2

FAM38A/PIEZO1 Mouse Monoclonal Antibody [2-10]

cat.: M1005-2

Product Type:	Mouse monoclonal IgG2a, primary antibodies
Species reactivity:	Human, Mouse, Rat
Applications:	WB, IF-Cell, IHC-P
Clonality:	Monoclonal
Clone number:	2-10

Form:	Liquid
Storage condition:	Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer:	1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration:	2ug/ul
Purification:	Protein A affinity purified.
Molecular weight:	Predicted band size: 287 kD
Isotype:	IgG2a
Immunogen:	Recombinant protein within Human Protein FAM38A aa 1275-1540 / 2521.
Positive control:	MCF-7, Hela, Siha, A431, mouse brain tissue, rat brain tissue, rat hippocampus tissue.
Subcellular location:	Endoplasmic reticulum membrane. Cell membrane.
Recommended Dilutions: WB IF-Cell IHC-P	1:500-1:2,000 1:100 1:200-1:600
Uniprot #:	SwissProt: Q92508 Human \| E2JF22 Mouse \| Q0KL00 Rat
Alternative names:	DHS Fam38a Family with sequence similarity 38 member A KIAA0233 Membrane protein induced by beta-amyloid treatment Mib PIEZ1_HUMAN Piezo-type mechanosensitive ion channel component 1 PIEZO1 Protein FAM38A Protein FAM38B Protein PIEZO1

Images

	Fig1: Western blot analysis of FAM38A/PIEZO1 on recombinant protein with Mouse anti-FAM38A/PIEZO1 antibody (M1005-2) at 1/500 dilution. Lysates/proteins at 50 ng/Lane. Exposure time: 1 minute; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1005-2) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature.
	Fig2: Western blot analysis of FAM38A/PIEZO1 on MCF-7 cell lysates with Mouse anti-FAM38A/PIEZO1 antibody (M1005-2) at 1/500 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 287 kDa Observed band size: 287 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1005-2) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature.
	Fig3: Immunocytochemistry analysis of Hela cells labeling FAM38A/PIEZO1 with Mouse anti-FAM38A/PIEZO1 antibody (M1005-2) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% BSA for 30 minutes at room temperature. Cells were then incubated with Mouse anti-FAM38A/PIEZO1 antibody (M1005-2) at 1/100 dilution in 2% BSA overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

	Fig4: Immunocytochemistry analysis of Siha cells labeling FAM38A/PIEZO1 with Mouse anti-FAM38A/PIEZO1 antibody (M1005-2) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% BSA for 30 minutes at room temperature. Cells were then incubated with Mouse anti-FAM38A/PIEZO1 antibody (M1005-2) at 1/100 dilution in 2% BSA overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
	Fig5: Immunocytochemistry analysis of A431 cells labeling FAM38A/PIEZO1 with Mouse anti-FAM38A/PIEZO1 antibody (M1005-2) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% BSA for 30 minutes at room temperature. Cells were then incubated with Mouse anti-FAM38A/PIEZO1 antibody (M1005-2) at 1/100 dilution in 2% BSA overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
	Fig6: Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Mouse anti-FAM38A/PIEZO1 antibody (M1005-2) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (M1005-2) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Fig7: Immunohistochemical analysis of paraffin-embedded rat brain tissue with Mouse anti-FAM38A/PIEZO1 antibody (M1005-2) at 1/600 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (M1005-2) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Fig8: Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue with Mouse anti-FAM38A/PIEZO1 antibody (M1005-2) at 1/600 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (M1005-2) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.