CD80 Mouse Monoclonal Antibody [8-E5]
cat.: M1007-10
| Product Type: | Mouse monoclonal IgG2b, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | 8-E5 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 33 kDa |
| Isotype: | IgG2b |
| Immunogen: | Synthetic peptide within human CD80 aa 74-118 / 288. |
| Positive control: | Human brain tissue lysates, human brain tissue, Raji. |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
WB IHC-P FC |
1:1,000 1:50 1:1,000 |
| Uniprot #: | SwissProt: P33681 Human | Q00609 Mouse |
| Alternative names: | Activation B7-1 antigen B lymphocyte activation antigen B7 B7 B7-1 B7-1 antigen B7.1 BB1 CD28 antigen ligand 1 CD28LG CD28LG1 CD80 CD80 antigen (CD28 antigen ligand 1, B7-1 antigen) CD80 antigen CD80 molecule CD80_HUMAN Costimulatory factor CD80 costimulatory molecule variant IgV-CD80 CTLA-4 counter-receptor B7.1 LAB7 T-lymphocyte activation antigen CD80 |
Images
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Fig1:
Western blot analysis of CD80 on human brain tissue lysates with Mouse anti-CD80 antibody (M1007-10) at 1/1,000 dilution. Lysates/proteins at 40 µg/Lane. Predicted band size: 33 kDa Observed band size: 60 kDa Exposure time: 3 minutes 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1007-10) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2: Immunohistochemical analysis of paraffin- embedded human brain tissue using using anti-CD80 Mouse mAb (Cat. # M1007-10). |
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Fig3:
Flow cytometric analysis of HUVEC (left, negative) and Raji (right, positive) cells labeling CD80. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (M1007-10, 1/1,000) (red) compared with Mouse IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody (HA1125) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.