HRP Conjugated Mouse anti-Human IgG light chain(kappa chain) Mouse Monoclonal Antibody [A11-G11]
cat.: M1007-11
| Product Type: | Mouse monoclonal IgG2a, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, ELISA |
| Clonality: | Monoclonal |
| Clone number: | A11-G11 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. |
| Concentration: | 2ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 25 kDa |
| Isotype: | IgG2a |
| Immunogen: | Human IgG light chain |
| Positive control: | Human IgG |
| Subcellular location: | Serum/ Plasma |
| Recommended Dilutions:
WB ELISA |
1:2,000-1:5,000 1:1,000 |
Images
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Fig1: Representative standard curve using Mouse mAb (Cat. # M1007-11). |
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Fig2:
Western blot analysis of Mouse anti-Human IgG light chain(kappa chain) on different lysates with Mouse anti-Human IgG light chain(kappa chain) antibody (M1007-11) at 1/2,000 dilution. Lane 1: Human IgG kappa light chain Lane 2: Rabbit IgG (negative) Lysates/proteins at 50 ng/Lane. Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1007-11) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. |
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Fig3: Western blot analysis on human IgG κ chain using anti-Human IgG light chain(kappa chain) conjugated HRP Mouse mAb (Cat. # M1007-11). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.