C12orf51 Mouse Monoclonal Antibody [A2-F5-F10]
cat.: M1007-22
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IHC-P, IF-Cell, FC |
| Clonality: | Monoclonal |
| Clone number: | A2-F5-F10 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 438/145/98/262 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within Human Protein C12orf51 aa 232-516 / 3,996. |
| Positive control: | HepG2, A549, SH-SY5Y, PC-3M, human colon cancer tissue, human kidney tissue, mouse testis tissue. |
| Subcellular location: | Membrane. Single-pass membrane protein. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:500 1:50 1:50-1:100 1:50-1:100 |
| Uniprot #: | SwissProt: Q9Y4D8 Human | Q6PDJ9 Mouse |
| Alternative names: | C12orf51 HECT domain-containing protein 4 HECTD4 KIAA0614 Probable E3 ubiquitin-protein ligase HECTD4 |
Images
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Fig1: Western blot analysis of C12orf51 on SH-SY5Y (1) and A549 (2) using anti-C12orf51 antibody at 1/100 dilution. |
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Fig2: ICC staining C12orf51 (green) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining C12orf51 (green) in PC-3M cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-C12orf51 antibody. Counter stained with hematoxylin. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-C12orf51 antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-C12orf51 antibody. Counter stained with hematoxylin. |
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Fig7: Flow cytometric analysis of SH-SY5Y cells with C12orf51 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.