Lactate Dehydrogenase A Mouse Monoclonal Antibody [20-179]
cat.: M1007-7
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | IHC-P |
| Clonality: | Monoclonal |
| Clone number: | 20-179 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 37 kDa |
| Isotype: | IgG1 |
| Immunogen: | Synthetic peptide within Human LDH-A aa 1-50 / 332. |
| Positive control: | Mouse testis tissue, human kidney tissue. |
| Subcellular location: | Cytoplasm |
| Recommended Dilutions:
IHC-P |
1:50-1:100 |
| Uniprot #: | SwissProt: P00338 Human | P06151 Mouse |
| Alternative names: | Cell proliferation-inducing gene 19 protein GSD11 L lactate dehydrogenase A chain L-lactate dehydrogenase A chain l7R2 Lactate dehydrogenase 1, A chain Lactate dehydrogenase A Lactate dehydrogenase A4 Lactate dehydrogenase M LDH A LDH M LDH muscle subunit LDH muscle subunit; M LDH LDH-A LDH-M LDH1 ldha LDHA_HUMAN LDHM OTTMUSP00000017774 PIG19 Proliferation-inducing gene 19 Renal carcinoma antigen NY-REN-59 |
Images
|
Fig1: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Lactate Dehydrogenase A antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1007-7, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Mouse anti-Lactate Dehydrogenase A antibody (M1007-7) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1007-7) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.