HSP60 Mouse Monoclonal Antibody [9-49]
cat.: M1007-9
Product Type: Mouse monoclonal IgG2a, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IHC-P, FC
Clonality: Monoclonal
Clone number: 9-49
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 2ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 61kDa
Isotype: IgG2a
Immunogen: Synthetic peptide within Human HSP60 aa 301-350 / 573.
Positive control: Hela, HepG2, human kidney tissue, mouse kidney tissue, human lung cancerr tissue.
Subcellular location: Mitochondrion matrix
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
1:500-1;1,000
1:100
1:50-400
Uniprot #: SwissProt: P10809 Human | P63038 Mouse | P63039 Rat
Alternative names: 60 kDa chaperonin 60 kDa heat shock protein, mitochondrial CH60_HUMAN Chaperonin 60 Chaperonin, 60-KD CPN60 fa04a05 GROEL heat shock 60kDa protein 1 (chaperonin) Heat shock protein 1 (chaperonin) Heat shock protein 60 Heat shock protein 65 heat shock protein family D (Hsp60) member 1 HLD4 Hsp 60 HSP 65 HSP-60 HSP60 HSP65 HSPD1 HuCHA60 Mitochondrial matrix protein P1 P60 lymphocyte protein short heat shock protein 60 Hsp60s1 SPG13
Images
M1007-9_1.jpg Fig1: Western blot analysis on Hela lysates using anti-HSP60 Mouse mAb (Cat. # M1007-9).
M1007-9_2.png Fig2: Immunocytochemistry analysis of Hela cells labeling HSP60 with Mouse anti-HSP60 antibody (M1007-9) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-HSP60 antibody (M1007-9) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
M1007-9_3.png Fig3: Immunocytochemistry analysis of HepG2 cells labeling HSP60 with Mouse anti-HSP60 antibody (M1007-9) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-HSP60 antibody (M1007-9) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
M1007-9_4.jpg Fig4: Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using anti-HSP60 Mouse mAb (Cat. # M1007-9).
M1007-9_5.png Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue with Mouse anti-HSP60 antibody (M1007-9) at 1/100 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1007-9) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
M1007-9_6.png Fig6: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Mouse anti-HSP60 antibody (M1007-9) at 1/100 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1007-9) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
M1007-9_7.png Fig7: Immunohistochemical analysis of paraffin-embedded human lung cancerr tissue with Mouse anti-HSP60 antibody (M1007-9) at 1/400 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1007-9) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.