HA tag Recombinant Mouse Monoclonal Antibody [2-G10]
cat.: M1008-1
| Product Type: | Recombinant Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Species independent |
| Applications: | WB, IHC-P, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | 2-G10 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG1 |
| Immunogen: | Synthetic peptide corresponding to HA tag conjugated to KLH. |
| Recommended Dilutions:
WB IHC-P IF-Cell |
1:10,000-1:25,000 1:1,000-1:10,000 1:1,000 |
| Alternative names: | HA epitope tag HA1 HA2 hemagglutinin Hemagglutinin HA1 chain Hemagglutinin HA2 chain |
Images
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Fig1:
Western blot analysis of HA tag on different lysates with Mouse anti-HA tag antibody (M1008-1) at 1/10,000 dilution. Lane 1: 293T cell lysate Lane 2: 293T transfected with HA-tagged Nanos homolog 3 (N-terminal) cell lysate Lane 3: 293T transfected with HA-tagged LIPT1 (N-terminal) cell lysate Lysates/proteins at 10 µg/Lane. Exposure time: 4 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1008-1) at 1/10,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of HA tag on different lysates with Mouse anti-HA tag antibody (M1008-1) at 1/25,000 dilution. Lane 1: 293T cell lysate (10 µg/Lane) Lane 2: 293T transfected with HA-tagged Nanos homolog 3 (N-terminal) cell lysate (10 µg/Lane) Lane 3: 293T transfected with HA-tagged CLK4 (C-terminal) cell lysate (20 µg/Lane) Exposure time: 1minute 50 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1008-1) at 1/25,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded 293T cells transfected with HA-tagged CLK4 (C-terminal) with Mouse anti-HA tag antibody (M1008-1) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1008-1) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded 293T cells transfected with HA-tagged Nanos homolog 3 (N-terminal) with Mouse anti-HA tag antibody (M1008-1) at 1/10,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1008-1) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunocytochemistry analysis of 293T cells labeling HA tag with Mouse anti-HA tag antibody (M1008-1) at 1/1,000 dilution. 293T cells, transfected with empty control (upper, negative) / HA-tagged Nanos homolog 3 (N-terminal) (lower, positive) expression vector, respectively, were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-HA tag antibody (M1008-1) at 1/1,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.