HA tag Mouse Monoclonal Antibody [2-G10]
cat.: M1008-1
| Product Type: | Mouse monoclonal IgM, primary antibodies |
|---|---|
| Species reactivity: | Species independent |
| Applications: | WB, IP, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | 2-G10 |
| Form: | Liquid |
| Storage condition: | Store at -20℃. Stable for 12 months from date of receipt. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein G affinity purified. |
| Isotype: | IgM |
| Immunogen: | Synthetic peptide corresponding to HA tag conjugated to KLH. |
| Positive control: | Recombinant protein with HA tag, HA-Gag transfected Hela cells. |
| Recommended Dilutions:
WB IP IF-Cell |
1:500-1:1,000 2-5 µg/ml. 1:100 |
| Alternative names: | HA epitope tag HA1 HA2 hemagglutinin Hemagglutinin HA1 chain Hemagglutinin HA2 chain |
Images
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Fig1: Western blot analysis on recombinant protein with HA tag using anti-HA tag Mouse mAb (Cat. # M1008-1). |
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Fig2:
HA tag was immunoprecipitated in 5µg C terminal HA Tag fusion protein lysate with M1008-1 at 2 µg/20 µl agarose. Western blot was performed from the immunoprecipitate using ET1611-49 at 1/500 dilution. Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 60 mins at room temperature. Lane 1: HA Tag fusion protein lysate (input). Lane 2: M1008-1 IP in HA Tag fusion protein lysate. Lane 3: Mouse IgG instead of M1008-1 in HA Tag fusion protein lysate. Blocking/Dilution buffer: 5% NFDM/TBST |
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Fig3:
Immunocytochemistry analysis of HA-Tag transfected Hela cells labeling HA tag with Mouse anti-HA tag antibody (M1008-1) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% BSA for 30 minutes at room temperature. Cells were then incubated with Mouse anti-HA tag antibody (M1008-1) at 1/100 dilution in 2% BSA overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.