FAM3A Mouse Monoclonal Antibody [7-B9]
cat.: M1008-10
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | 7-B9 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 25 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within Human FAM3A 1-230 / 230. |
| Positive control: | Rat smooth muscle tissue lysates, human kidney tissue, human fetal skeletal muscle tissue, mouse heart tissue, A431. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB IHC-P FC |
1:2,000-1:5,000 1:50-1:200 1:50-1:200 |
| Uniprot #: | SwissProt: P98173 Human | Q9D8T0 Mouse Entrez Gene: 501664 Rat |
| Alternative names: | 2-19 2.19 Cytokine-like protein 2-19 DLD DXS560S FAM3A FAM3A_HUMAN family with sequence similarity 3, member A Protein FAM3A XAP 7 |
Images
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Fig1:
Western blot analysis of FAM3A on rat smooth muscle tissue lysates with Mouse anti-FAM3A antibody (M1008-10) at 1/500 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 25 kDa Observed band size: 25 kDa Exposure time: 30 seconds; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1008-10) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature. |
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Fig2: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-FAM3A antibody. Counter stained with hematoxylin. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human fetal skeletal muscle tissue using anti-FAM3A antibody. Counter stained with hematoxylin. |
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Fig4: Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-FAM3A antibody. Counter stained with hematoxylin. |
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Fig5: Flow cytometric analysis of A431 cells with FAM3A antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.