V5 tag Mouse Monoclonal Antibody [A10-D0-A1]
cat.: M1008-2
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Species independent |
| Applications: | WB, ELISA, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | A10-D0-A1 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein G affinity purified. |
| Isotype: | IgG1 |
| Immunogen: | Peptide GKPIPNPLLGLDST (V5 epitope) conjugated with KLH. |
| Positive control: | V5-tag recombinant protein. |
| Recommended Dilutions:
WB ELISA IF-Cell |
1:5,000-1:10,000 1:20,000-1:50,000 1:1,000 |
| Alternative names: | GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST tag Protein Rev Regulator of expression of viral proteins rev V5 epitope tag |
Images
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Fig1:
Western blot analysis of V5 tag on different lysates with Mouse anti-V5 tag antibody (M1008-2) at 1/5,000 dilution. Lane 1: 293T cell lysate Lane 2: 293T transfected with V5-tagged Histone H3.1 (N-terminal) cell lysate Lane 3: 293T transfected with V5-tagged Nanos homolog 3 (C-terminal) cell lysate Lysates/proteins at 10 µg/Lane. Exposure time: 2 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1008-2) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of HeLa cells labeling V5 tag with Mouse anti-V5 tag antibody (M1008-2) at 1/1,000 dilution. HeLa cells, transfected with V5-tagged empty control, Histone H3 (N-terminal) or Nanos homolog 3 (C-terminal) expression vector, respectively, were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-V5 tag antibody (M1008-2) at 1/1,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. V5 tag (ET1605-41, green) was stained at 1/2,000 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.