| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IF-Cell, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | 4-B3 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 54 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within Human Transmembrane protein 200A aa 59-359 / 491. |
| Positive control: | HepG2, A549, F9, NCCIT, human pancreas tissue. |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:500 1:100 1:50 |
| Uniprot #: | SwissProt: Q86VY9 Human | Q8C817 Mouse |
| Alternative names: | KIAA1913 T200A_HUMAN Tmem200a Transmembrane protein 200A TTMC Two transmembrane C |
|
Fig1: Western blot analysis of Transmembrane protein 200A on A549 (1) and HepG2 (2) using anti-Transmembrane protein 200A antibody at 1/500 dilution. |
|
Fig2: ICC staining Transmembrane protein 200A (red) in F9 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
|
Fig3: ICC staining Transmembrane protein 200A (green) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
|
Fig4: ICC staining Transmembrane protein 200A (red) in NCCIT cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
|
Fig5: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-Transmembrane protein 200A antibody. Counter stained with hematoxylin. |