Product Type: | Mouse monoclonal IgG1, primary antibodies |
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Species reactivity: | Human, Mouse |
Applications: | WB, IF-Cell, IHC-P |
Clonality: | Monoclonal |
Clone number: | 4-B3 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 2ug/ul |
Purification: | Immunogen affinity purified. |
Molecular weight: | Predicted band size: 54 kDa |
Isotype: | IgG1 |
Immunogen: | Recombinant protein within Human Transmembrane protein 200A aa 59-359 / 491. |
Positive control: | HepG2, A549, F9, NCCIT, human pancreas tissue. |
Subcellular location: | Membrane. |
Recommended Dilutions:
WB IF-Cell IHC-P |
1:500 1:100 1:50 |
Uniprot #: | SwissProt: Q86VY9 Human | Q8C817 Mouse |
Alternative names: | KIAA1913 T200A_HUMAN Tmem200a Transmembrane protein 200A TTMC Two transmembrane C |
Fig1: Western blot analysis of Transmembrane protein 200A on A549 (1) and HepG2 (2) using anti-Transmembrane protein 200A antibody at 1/500 dilution. | |
Fig2: ICC staining Transmembrane protein 200A (red) in F9 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. | |
Fig3: ICC staining Transmembrane protein 200A (green) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. | |
Fig4: ICC staining Transmembrane protein 200A (red) in NCCIT cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
Fig5: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-Transmembrane protein 200A antibody. Counter stained with hematoxylin. |