Transmembrane protein 200A Mouse Monoclonal Antibody [4-B3]
cat.: M1008-8
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IF-Cell, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | 4-B3 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 54 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within Human Transmembrane protein 200A aa 59-359 / 491. |
| Positive control: | HepG2, A549, F9, NCCIT, human pancreas tissue. |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:500 1:100 1:50 |
| Uniprot #: | SwissProt: Q86VY9 Human | Q8C817 Mouse |
| Alternative names: | KIAA1913 T200A_HUMAN Tmem200a Transmembrane protein 200A TTMC Two transmembrane C |
Images
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Fig1: ICC staining Transmembrane protein 200A (red) in F9 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig2: ICC staining Transmembrane protein 200A (green) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining Transmembrane protein 200A (red) in NCCIT cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-Transmembrane protein 200A antibody. Counter stained with hematoxylin. |
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Fig5:
Western blot analysis of Transmembrane protein 200A on different lysates with Mouse anti-Transmembrane protein 200A antibody (M1008-8) at 1/1,000 dilution. Lane 1: A549(Human lung cancer cells) cell lysate Lane 2: HepG2 (Human liver cancer cells) cell lysate Lane 3: Caco-2 (Human colon adenocarcinoma cells) cell lysate Lane 4: PC-3M (Human prostate cancer cells) cell lysate Lysates/proteins at 20 µg/Lane. Exposure time: 45 seconds; ECL: K1802 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: M1008-8, 1/1,000 in primary antibody dilution buffer 5% NFDM/TBST, overnight at 4 ℃ Secondary antibody: Goat anti-Mouse IgG-HRP (HA1006), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 54.4 kDa Observed band size: 47 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.