| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | IHC-P |
| Clonality: | Monoclonal |
| Clone number: | C4-D0 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein G affinity purified. |
| Molecular weight: | Predicted band size: 102 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within human FAM62B aa 300-550. |
| Positive control: | Human colon cancer tissue, human kidney tissue. |
| Subcellular location: | Cell membrane, Endoplasmic reticulum, Membrane. |
| Recommended Dilutions:
IHC-P |
1:200 |
| Uniprot #: | SwissProt: A0FGR8 Human |
| Alternative names: | Chr2 synaptotagmin CHR2SYT E Syt2 ESYT 2 ESYT2 Extended synaptotagmin 2 Extended synaptotagmin like protein 2 FAM 62B Family with sequence similarity 62 (C2 domain containing) member B Family with sequence similarity 62 member B KIAA1228 Protein FAM62B |
|
Fig1:
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Mouse anti-FAM62B antibody (M1009-1) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1009-1) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Mouse anti-FAM62B antibody (M1009-1) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1009-1) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |