FAM62B Mouse Monoclonal Antibody [C4-D0]
cat.: M1009-1
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | C4-D0 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein G affinity purified. |
| Molecular weight: | 102 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within human FAM62B aa 300-550. |
| Positive control: | Siha cell lysate, THP-1 cell lysate, human colon carcinoma tissue. |
| Subcellular location: | Cell membrane, Endoplasmic reticulum, Membrane. |
| Recommended Dilutions:
WB IHC-P |
1:500 1:50-1:200 |
| Uniprot #: | SwissProt: A0FGR8 Human |
| Alternative names: | Chr2 synaptotagmin CHR2SYT E Syt2 ESYT 2 ESYT2 Extended synaptotagmin 2 Extended synaptotagmin like protein 2 FAM 62B Family with sequence similarity 62 (C2 domain containing) member B Family with sequence similarity 62 member B KIAA1228 Protein FAM62B |
Images
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Fig1:
Western blot analysis of FAM62B on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (M1009-1, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Siha cell lysate Lane 2: THP-1 cell lysate |
|
Fig2: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-FAM62B antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1009-1, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.