C14orf93 Mouse Monoclonal Antibody [2-F6]
cat.: M1009-3
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IF-Cell, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | 2-F6 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 59 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within Human C14orf93 aa 1-263 / 538. |
| Positive control: | C14orf93-GST recombinant protein lysate, HepG2, mouse skin tissue, human spleen tissue, human colon cancer tissue, human thyroid tissue. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:500-1:1,000 1:50-1:200 1:50-1:200 |
| Uniprot #: | SwissProt: Q9H972 Human | Q8K2W9 Mousse |
| Alternative names: | C14orf93 CN093_HUMAN Uncharacterized protein C14orf93 Uncharacterized protein C14orf93 homolog |
Images
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Fig1: Western blot analysis of C14orf93 on C14orf93-GST recombinant protein lysate using anti-C14orf93 antibody at 1/1,000 dilution. |
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Fig2:
Immunocytochemistry analysis of HepG2 cells labeling C14orf93 with Mouse anti-C14orf93 antibody (M1009-3) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-C14orf93 antibody (M1009-3) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded mouse skin tissue with Mouse anti-C14orf93 antibody (M1009-3) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1009-3) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-C14orf93 antibody. Counter stained with hematoxylin. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-C14orf93 antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded human thyroid tissue using anti-C14orf93 antibody. Counter stained with hematoxylin. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.