FAM234A Mouse Monoclonal Antibody [A5-A10]
cat.: M1010-1
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | A5-A10 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein G affinity purified. |
| Molecular weight: | Predicted band size: 60 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within human FAM234A aa 300-552. |
| Positive control: | SiHa cell lysate, SH-SY5Y cell lysate, NIH:OVCAR-3 cell lysate, A549 cell lysate, Neuro-2a cell lysate, C6 cell lysate, LOVO. |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
WB IF-Cell |
1:500-1:2,000 1:50-1:100 |
| Uniprot #: | SwissProt: Q9H0X4 Human | Q8C0Z1 Mouse | Q5M7W6 Rat |
| Alternative names: | Protein ITFG3 Protein FAM234A C16orf9 ITFG3 FAM234A |
Images
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Fig1:
Western blot analysis of FAM234A on different lysates with Mouse anti-FAM234A antibody (M1010-1) at 1/2,000 dilution. Lane 1: SiHa cell lysate Lane 2: SH-SY5Y cell lysate Lane 3: NIH:OVCAR-3 cell lysate Lane 4: A549 cell lysate Lane 5: Neuro-2a cell lysate Lane 6: C6 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 60 kDa Observed band size: 55 kDa Exposure time: 2 minutes 5 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1010-1) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2: ICC staining of FAM234A in LOVO cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (M1010-1, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.